Hasanin Mohamed S, Emam Mahmoud, Ahmed M A, Rohim F M, Mohamed M A A, El Saied Housni, Hussein Hamdy A Z, Abdelkhalek A
Cellulose & Paper Department, National Research Centre, El-Buhouth St., Dokki, Cairo, 12622, Egypt.
Phytochemistry and Plant Systematics Department, National Research Centre, Dokki, Cairo, 12622, Egypt.
Bioresour Bioprocess. 2025 Jun 6;12(1):54. doi: 10.1186/s40643-025-00890-9.
Storage of economic fruits is a fatal economic and nutritional factor for most countries. Edible coating played a restricted role in this manner, forcing good usability with many limitations. In this work, Apple (Malus domestica var. Anna) was coated using bioactive nanopackaging films formulated to overcome the economic limitations and drawbacks of conventional coating. The formulated bioactive nanopackaging was based on nanochitosan prepared from shrimp shells and orange peel waste, which is used to produce nanocellulose (white part) and extract active ingredients (orange part). The formulated bioactive nanopackaging based on nanochitosan and nanocellulose (2:1) and orange peel waste extract with ratios of 1, 3, and 5% (w/w) based on nanochitosan dry weight and called T3, T4, and T5, respectively. Characteristics of bioactive nanopackaging films and their precursor materials were characterized physicochemically and topographically as well. The waste orange peel waste extract was characterized phytochemically. According to the orange peel extract, the formulated bioactive nanopackaging films observed antioxidant and antimicrobial activity. The results revealed that all treatments outperformed the control, especially treatments T4 (1% Nano Chitosan + 1% Nano Cellulose + 3% Orange Peel Waste extract) and T5 (1% Nano Chitosan + 1% Nano Cellulose + 5% Orange Peel Waste extract), in terms of fruit decay percentage (11.72 ± 11.4 C and 12.33 ± 10.83 C, respectively), weight loss (3.81 ± 2.29B and 3.77 ± 2.22B, respectively), TSS/acidity (17.07 ± 1.14 A and 16.77 ± 1.18 A, respectively), fruit firmness (12.83 ± 1.19B and 13.48 ± 0.91 A, respectively), total sugars (7.98 ± 0.21 A and 8.21 ± 0.29 A, respectively) and total anthocyanin (0.21 ± 0.03B and 0.25 ± 0.05 A, respectively).
对于大多数国家而言，经济水果的储存是一个至关重要的经济和营养因素。可食用涂层在这方面发挥了一定的限制作用，虽然具有一定可用性，但也存在诸多局限。在本研究中，使用生物活性纳米包装薄膜对苹果（苹果品种安娜）进行包衣处理，以克服传统涂层的经济局限性和缺点。所制备的生物活性纳米包装材料基于从虾壳和橙皮废料中制备的纳米壳聚糖，用于生产纳米纤维素（白色部分）并提取活性成分（橙色部分）。基于纳米壳聚糖和纳米纤维素（2:1）以及分别按纳米壳聚糖干重的1%、3%和5%（w/w）比例添加橙皮废料提取物制备的生物活性纳米包装材料，分别称为T3、T4和T5。对生物活性纳米包装薄膜及其前驱体材料的特性进行了物理化学和形貌表征。对橙皮废料提取物进行了植物化学表征。根据橙皮提取物，所制备的生物活性纳米包装薄膜具有抗氧化和抗菌活性。结果表明，所有处理组均优于对照组，尤其是处理组T4（1%纳米壳聚糖 + 1%纳米纤维素 + 3%橙皮废料提取物）和T5（1%纳米壳聚糖 + 1%纳米纤维素 + 5%橙皮废料提取物），在果实腐烂率（分别为11.72±11.4C和12.33±10.83C）、失重率（分别为3.81±2.29B和3.77±2.22B）、总可溶性固形物/酸度（分别为17.07±1.14A和16.77±1.18A）、果实硬度（分别为12.83±1.19B和13.48±0.91A）、总糖含量（分别为7.98±0.21A和8.21±0.29A）以及总花青素含量（分别为0.21±0.03B和0.25±0.05A）方面。
