Pryor G T, Howd R A, Uyeno E T, Thurber A B
Pharmacol Biochem Behav. 1985 Sep;23(3):401-10. doi: 10.1016/0091-3057(85)90013-9.
Weanling male Fischer-344 rats were exposed by inhalation to air or 2000 ppm toluene for 8 hours each day for 2 weeks. Subgroups had access to water or 6% alcohol as their only fluid sources, respectively. Rats exposed to both toluene and alcohol subsequently showed a marked preference for 6% alcohol in two-bottle choice tests that persisted for up to 20 days for some rats. Rats exposed to toluene without access to alcohol and control rats (exposed to air and water) showed a marked aversion to the alcohol solution, and only 2 of 12 rats forced to drink alcohol without exposure to toluene preferred alcohol in the preference tests. Exposure to both toluene and alcohol also caused greater inhibition of weight gain than exposure to either substance alone, accompanied by greater signs of organ toxicity as indicated by clinical blood chemistries. Exposure to toluene caused marked hearing loss as assessed by a behavioral technique (conditioned avoidance), and there was a trend toward enhancement of this ototoxic effect by forced consumption of alcohol.
将断奶雄性Fischer - 344大鼠每天吸入空气或2000 ppm甲苯8小时，持续2周。各亚组分别以水或6%酒精作为唯一的液体来源。随后，暴露于甲苯和酒精的大鼠在双瓶选择试验中对6%酒精表现出明显的偏好，部分大鼠这种偏好持续长达20天。暴露于甲苯但无酒精的大鼠和对照大鼠（暴露于空气和水）对酒精溶液表现出明显的厌恶，在偏好试验中，12只未暴露于甲苯而被迫饮酒的大鼠中只有2只偏好酒精。与单独暴露于任何一种物质相比，同时暴露于甲苯和酒精还导致体重增加受到更大抑制，临床血液化学指标显示器官毒性迹象更明显。通过行为技术（条件回避）评估，暴露于甲苯会导致明显的听力损失，并且强迫饮酒有增强这种耳毒性作用的趋势。
