Robinson D R, Prickett J D, Polisson R, Steinberg A D, Levine L
Prostaglandins. 1985 Jul;30(1):51-75. doi: 10.1016/s0090-6980(85)80010-1.
Dietary marine lipids markedly reduce the severity of glomerulonephritis and its associated mortality in inbred strains of mice developing autoimmune disease, a model for human systemic lupus erythematosus. We report here the influence of varying the dose of menhaden oil and the timing of its administration on the mortality of female (NZB x NZW) F1 mice. After ingesting 25 wt% menhaden oil (MO) for periods of 1.5 weeks to 12 months, there was a stable content of tissue n-3 fatty acids, with total n-3 fatty acids of 28% and 35% in spleen and liver, respectively. The extent of protection from mortality was dependent on the dose of MO with marked protection at doses of 11 to 25%, marginal protection at 5.5% and no protection at 2.5% MO. Delay in the institution of MO until ages 5 or 7 months still resulted in large reductions of mortality. Conversely, institution of a MO diet from 6 weeks until ages 5 to 7 months followed by a change to beef tallow resulted in little protection. Serum levels of 4 cyclooxygenase products were reduced ranging from 26 to 76% in mice fed MO diets, compared to mice fed beef tallow, based on radioimmunoassay. The degree of reduction of mortality on different doses of MO was correlated best with tissue levels of C22:5, and levels of C20:5 and C22:6 were similar at high and low doses of MO, suggesting that levels of 22:5 may be related to the protective effects of marine lipids on autoimmune disease.
在患有自身免疫性疾病的近交系小鼠（一种人类系统性红斑狼疮的模型）中，膳食海洋脂质可显著降低肾小球肾炎的严重程度及其相关死亡率。我们在此报告了不同剂量的鲱鱼油及其给药时间对雌性（NZB×NZW）F1小鼠死亡率的影响。在摄入25%重量比的鲱鱼油（MO）1.5周至12个月后，组织中n-3脂肪酸含量稳定，脾脏和肝脏中的总n-3脂肪酸分别为28%和35%。对死亡率的保护程度取决于MO的剂量，11%至25%的剂量有显著保护作用，5.5%的剂量有边际保护作用，2.5%的MO剂量则无保护作用。将MO的给药延迟至5或7个月龄仍可大幅降低死亡率。相反，从6周龄开始给予MO饮食直至5至7个月龄，然后改为牛脂饮食，几乎没有保护作用。基于放射免疫分析，与喂食牛脂的小鼠相比，喂食MO饮食的小鼠血清中4种环氧化酶产物水平降低了26%至76%。不同剂量MO对死亡率的降低程度与组织中C22:5水平的相关性最好，高剂量和低剂量MO下C20:5和C22:6的水平相似，这表明22:5的水平可能与海洋脂质对自身免疫性疾病的保护作用有关。
