Song Yunji, Kim Yeji, Kim Jihyun, Kim Ha-Jung
Department of Internal Medicine, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, South Korea.
BK 21 Project Team, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, South Korea.
One Health. 2025 May 16;20:101076. doi: 10.1016/j.onehlt.2025.101076. eCollection 2025 Jun.
Household endotoxin exposure and house dust mite (HDM) allergens have been recently gaining attention for their immunological potential to affect allergic symptoms. Although dogs are spontaneous models of human atopic dermatitis (AD), there are few studies exploring their relationship. The aim of this study is to analyze the effects of HDM and endotoxin concentrations on AD in dogs and compare their immunological profiles with humans.
We graded the canine AD Extent and Severity Index-4 (CADESI-4), Pruritus Visual Analogue Scale (PVAS), and transepidermal water loss (TEWL) in dogs with ( = 42) and without AD (controls; = 18). Serum immunoglobulin E (IgE), interleukin (IL)-4, IL-31, and IL-33 levels were measured. Dog owners were required to answer residential environmental questionnaires and measure the average indoor relative humidity (RH) and temperature for 48 h. Dust samples were collected from the floor and analyzed for HDM allergens including () and ) allergen and endotoxin.
Both the concentration and endotoxin exposure levels from the floor dust had an inverse association with TEWL in dogs with AD ( < 0.05) but not in controls. Furthermore, the endotoxin concentration was negatively associated with the IL-4 level. No significant difference was found in endotoxin exposure and the concentration between dogs with AD and controls.
Indoor HDM exposure is associated with decreased TEWL, and endotoxin concentrations may contribute to the mitigation of CAD symptoms.
家庭内毒素暴露和屋尘螨（HDM）过敏原因其影响过敏症状的免疫潜力最近受到关注。尽管狗是人类特应性皮炎（AD）的自发模型，但很少有研究探讨它们之间的关系。本研究的目的是分析HDM和内毒素浓度对犬AD的影响，并将它们的免疫特征与人类进行比较。
我们对患有AD（n = 42）和未患AD（对照组；n = 18）的犬进行了犬AD范围和严重程度指数-4（CADESI-4）、瘙痒视觉模拟量表（PVAS）和经表皮水分流失（TEWL）分级。检测血清免疫球蛋白E（IgE）、白细胞介素（IL）-4、IL-31和IL-33水平。要求狗主人回答居住环境问卷，并测量48小时内的室内平均相对湿度（RH）和温度。从地板上收集灰尘样本，分析其中的HDM过敏原，包括（）和）过敏原以及内毒素。
地板灰尘中的浓度和内毒素暴露水平与患有AD的犬的TEWL呈负相关（P < 0.05），但在对照组中无此相关性。此外，内毒素浓度与IL-4水平呈负相关。AD犬和对照组之间在内毒素暴露和浓度方面未发现显著差异。
室内HDM暴露与TEWL降低有关，内毒素浓度可能有助于减轻CAD症状。
