He Dan, Cheng Yuqiang, Lv Hongkai, Geng Anqi, Zheng Jie, Dang Lin, Li Pengfei
Laboratory of Animal Center, Medical Experiment Center, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi, China.
Research & Development Institute, Northwestern Polytechnical University, Shenzhen, Guangdong, China.
PeerJ. 2025 Jun 3;13:e19449. doi: 10.7717/peerj.19449. eCollection 2025.
Amnion-derived stem cells exhibit several significant advantages, including low immunogenicity, anti-inflammatory, anti-fibrotic, and angiogenic properties, which have garnered considerable attention as a potential source for cell therapy. Numerous studies have demonstrated that proteins secreted by amniotic stem cells play a crucial role in facilitating regenerative processes and reflect the therapeutic benefits across various diseases. Secreted protein products from stem cells offer solutions to challenges in cell therapy, such as improving efficacy during or intravenous administration. These products also hold significant clinical and commercial potential. Nonetheless, the establishment of stringent quality control standards for secreted proteins from both amniotic mesenchymal stem cells (AMSCs) and amniotic epithelial cells (AECs) continues to pose a significant challenge. In this study, the expression profiles of secreted proteins from AMSCs and AECs were comprehensively analyzed utilizing mass spectrometry-based proteomics. The 71 highly abundant proteins and their potential biological functions were further investigated. Moreover, we identified and confirmed 17 hub proteins. Notably, ANXA2 was observed to enhance the expression of pro-inflammatory cytokines in macrophages. These findings may inform quality control measures for secreted protein products derived from amniotic stem cells and provide valuable insights for future research on the functional aspects of the secreted proteome in amniotic stem cells.
羊膜来源的干细胞具有几个显著优势，包括低免疫原性、抗炎、抗纤维化和促血管生成特性，作为细胞治疗的潜在来源已引起广泛关注。众多研究表明，羊膜干细胞分泌的蛋白质在促进再生过程中发挥关键作用，并反映出对各种疾病的治疗益处。干细胞分泌的蛋白质产物为细胞治疗中的挑战提供了解决方案，比如提高体内或静脉给药时的疗效。这些产物还具有重大的临床和商业潜力。尽管如此，为羊膜间充质干细胞（AMSCs）和羊膜上皮细胞（AECs）分泌的蛋白质建立严格的质量控制标准仍然是一项重大挑战。在本研究中，利用基于质谱的蛋白质组学全面分析了AMSCs和AECs分泌蛋白的表达谱。进一步研究了71种高丰度蛋白质及其潜在生物学功能。此外，我们鉴定并确认了17种核心蛋白。值得注意的是，观察到膜联蛋白A2（ANXA2）可增强巨噬细胞中促炎细胞因子的表达。这些发现可能为羊膜干细胞分泌蛋白产物的质量控制措施提供依据，并为未来关于羊膜干细胞分泌蛋白质组功能方面的研究提供有价值的见解。
