Key Laboratory of Neuropsychiatric Drug Research of Zhejiang Province, School of Pharmaceutical Sciences, Hangzhou Medical College, Hangzhou, 310013, Zhejiang, China.
Zhejiang Key Laboratory of Cardiovascular Intervention and Precision Medicine of Sir Run Run Shaw Hospital, Zhejiang University-Lishui Joint Innovation Center for Life and Health, Zhejiang University, Hangzhou, 310058, China.
Sci Rep. 2024 Nov 14;14(1):28061. doi: 10.1038/s41598-024-79063-1.
Perinatal stem cells have prominent applications in cell therapy and regenerative medicine. Among them, human Wharton's jelly mesenchymal stem/stromal cells (hWJMSCs) and human amniotic epithelial stem cells (hAESCs) have been widely used. However, the distinction in the therapeutic potential of hWJMSCs and hAESCs is poorly understood. In this study, we reported the phenotypic differences between these two distinct cell types and provided the first systematic comparison of their therapeutic potential in terms of immunomodulation, extracellular matrix (ECM) remodelling, angiogenesis and antioxidative stress using proteomics. The results revealed that the two cell types presented different protein expression profiles and were both promising candidates for cell therapy. Both types of cells demonstrated angiogenic and antifibrotic potential, whereas hAESCs presented superior immunological tolerance and antioxidant properties, which were supported by a series of relevant in vitro assays. Our study provides clues for the selection of appropriate cell types for diverse indications in cell therapy, which contributes to the advancement of their clinical translation and application.
围产期中胚层干细胞在细胞治疗和再生医学中有突出的应用。其中，人脐带华通氏胶间充质干细胞（hWJMSCs）和人羊膜上皮干细胞（hAESCs）已被广泛应用。然而，hWJMSCs 和 hAESCs 的治疗潜力之间的区别尚未被很好地理解。在本研究中，我们报道了这两种不同细胞类型之间的表型差异，并使用蛋白质组学首次系统比较了它们在免疫调节、细胞外基质（ECM）重塑、血管生成和抗氧化应激方面的治疗潜力。结果表明，这两种细胞类型表现出不同的蛋白表达谱，均是细胞治疗有前途的候选者。两种类型的细胞均表现出血管生成和抗纤维化的潜力，而 hAESCs 表现出更好的免疫耐受性和抗氧化特性，这一系列相关的体外实验得到了支持。我们的研究为细胞治疗中不同适应症选择合适的细胞类型提供了线索，有助于推进其临床转化和应用。
