Li Xueya, Liu Dazhen, Han Song, Liu Yujia, Fan Yuanyang, Zhang Yu, Li Chunchang, Xia Zhenshan, Cui Lingbing, Cui Jing, Wen Jinghong, Yan Tao, Jiang Chuanjia, Jin Yongxin, Ren Qian, Liu Mingyang
State Key Laboratory of Medicinal Chemical Biology, College of Life, Nankai University, Tianjin, 300350, China.
Department of Urology, Department of Neurology, Department of Gynecology and Obstetrics, Tianjin Medical University General Hospital, Tianjin, 300052, China.
Adv Sci (Weinh). 2025 Sep;12(33):e03793. doi: 10.1002/advs.202503793. Epub 2025 Jun 9.
The rapid emergence of drug-resistant bacteria has outpaced the development of traditional antibiotics, necessitating the exploration of more effective therapeutic strategies. In this study, the design of a CuS multifunctional nanozyme, activated by near-infrared (NIR) light is presented, that demonstrates enhanced antibacterial activity. CuS is synthesized with varying defect structures by utilizing different templates, which substantially optimize its absorption to HO and lipopolysaccharides (LPS) molecules. This process generates an optimal electronic structure, producing efficient antibacterial activity through photodynamic and photothermal synergetic processes. Specifically, the CuS nanozyme with dual defects (VCu and VCuCuCuSSS) exhibits peroxidase-like (POD), catalase-like (CAT), and GSH-depletion properties, effectively inactivating drug-resistant bacteria such as Pseudomonas aeruginosa. Notably, in a mouse wound model infected with P. aeruginosa, the nanozyme demonstrates significant antibacterial efficacy, promoting wound healing under NIR light. This multifunctional CuS nanozyme presents a promising new strategy for combating drug-resistant bacterial infections.
耐药细菌的迅速出现已经超过了传统抗生素的研发速度，因此有必要探索更有效的治疗策略。在本研究中，我们展示了一种由近红外（NIR）光激活的硫化铜（CuS）多功能纳米酶的设计，该纳米酶具有增强的抗菌活性。通过使用不同的模板合成具有不同缺陷结构的CuS，这极大地优化了其对羟基自由基（HO）和脂多糖（LPS）分子的吸收。这一过程产生了最佳的电子结构，通过光动力和光热协同过程产生高效的抗菌活性。具体而言，具有双缺陷（VCu和VCuCuCuSSS）的CuS纳米酶表现出类过氧化物酶（POD）、类过氧化氢酶（CAT）和谷胱甘肽消耗特性，有效地使耐药细菌如铜绿假单胞菌失活。值得注意的是，在感染铜绿假单胞菌的小鼠伤口模型中，该纳米酶显示出显著的抗菌效果，在近红外光下促进伤口愈合。这种多功能CuS纳米酶为对抗耐药细菌感染提供了一种有前景的新策略。
