Zhu Xiaolong, Lu Wei, Li Ziyi, Xiao Jiamu, Gan Donglin, Jiang Xuefeng, Wang Mingqian
National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, 210023 Nanjing, China.
National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, 210023 Nanjing, China.
J Colloid Interface Sci. 2025 Dec;699(Pt 1):138125. doi: 10.1016/j.jcis.2025.138125. Epub 2025 Jun 11.
Diseases caused by bacterial infections significantly threaten human health. However, the overuse of conventional antibiotics has led to rising drug resistance, necessitating the development of alternative antimicrobial strategies. In this study, we designed and synthesized a dual single-atom FeC nanozyme supported on nitrogen-doped carbon (NC), incorporating Fe single-atom sites, Co single-atom sites, and Fe-Co dual-atom sites. Characterization and theoretical calculations confirmed that the catalytic performance of FeCo-NC was superior to those of single-atom Fe-NC and Co-NC. Photothermal experiments demonstrated FeCo-NC's excellent photothermal properties and high photothermal conversion efficiency. Chemodynamic studies revealed its ability to efficiently catalyze HO for generating reactive oxygen species, outperforming Fe-NC and Co-NC in catalytic kinetics. In vitro and in vivo experiments confirmed that FeCo-NC effectively eliminated bacteria against methicillin-resistant Staphylococcus aureus (MRSA) (99.36 %) and Escherichia coli (E. coli) (99.76 %) through the synergistic effects of photothermal therapy and chemodynamic therapy. Transcriptome analysis further showed that FeCo-NC altered bacterial gene expression related to growth, metabolism, and membrane structure, enhancing its antibacterial efficacy. Beyond its antimicrobial effects, FeCo-NC reduced inflammation at infected wound sites, promoted macrophage polarization, enhanced collagen deposition, and accelerated wound healing. This study presents a promising antibiotic-free strategy for the clinical treatment of infected wounds.
细菌感染引起的疾病严重威胁人类健康。然而，传统抗生素的过度使用导致耐药性不断上升，因此有必要开发替代抗菌策略。在本研究中，我们设计并合成了一种负载在氮掺杂碳（NC）上的双单原子FeC纳米酶，其中包含铁单原子位点、钴单原子位点和铁-钴双原子位点。表征和理论计算证实，FeCo-NC的催化性能优于单原子Fe-NC和Co-NC。光热实验表明FeCo-NC具有优异的光热性能和高光热转换效率。化学动力学研究表明其能够有效催化产生羟基自由基以生成活性氧，在催化动力学方面优于Fe-NC和Co-NC。体外和体内实验证实，FeCo-NC通过光热疗法和化学动力学疗法的协同作用有效消除了耐甲氧西林金黄色葡萄球菌（MRSA）（99.36%）和大肠杆菌（E. coli）（99.76%）。转录组分析进一步表明，FeCo-NC改变了与细菌生长、代谢和膜结构相关的基因表达，增强了其抗菌效果。除了抗菌作用外，FeCo-NC还减轻了感染伤口部位的炎症，促进巨噬细胞极化，增强胶原蛋白沉积，并加速伤口愈合。本研究为感染伤口的临床治疗提出了一种有前景的无抗生素策略。
