Doillon C J, Silver F H, Berg R A
Biomaterials. 1987 May;8(3):195-200. doi: 10.1016/0142-9612(87)90063-9.
We have previously shown that the presence of fibronectin (FN) and/or hyaluronic acid (HA) in a 3-dimensional type I collagen sponge enhances wound healing in vivo. In the present study the same material was used as a support for growth of fibroblasts in vitro. Using radiochemical techniques, scanning electron and light microscopy, the properties of fibroblasts cultured on the collagen sponge or on the sponge containing HA or FN have been compared with cultures grown on plastic dishes. Fibroblast replication and collagen synthesis were higher on plastic than on the collagen sponge. In the presence of HA or FN the entire thickness of the sponge was infiltrated by fibroblasts which rapidly replicated. The presence of HA or FN increased synthesis of collagen which was largely deposited around cells.
我们之前已经表明，在三维I型胶原海绵中存在纤连蛋白（FN）和/或透明质酸（HA）可增强体内伤口愈合。在本研究中，相同的材料被用作体外成纤维细胞生长的支架。使用放射化学技术、扫描电子显微镜和光学显微镜，将在胶原海绵或含有HA或FN的海绵上培养的成纤维细胞的特性与在塑料培养皿上生长的培养物进行了比较。成纤维细胞在塑料上的复制和胶原合成高于在胶原海绵上。在HA或FN存在的情况下，海绵的整个厚度被快速复制的成纤维细胞浸润。HA或FN的存在增加了胶原的合成，胶原大量沉积在细胞周围。
