Castle M C, Cooke W J
Ther Drug Monit. 1985;7(3):364-8. doi: 10.1097/00007691-198507030-00024.
A deficiency of vitamin E has been associated with a wide variety of pathological conditions, many of them involving neonates. Previously published methods for the quantitation of vitamin E require large sample volumes as well as extensive extraction and concentration procedures. The present work describes a method for the quantitation of vitamin E in small volumes (less than or equal to 50 microliters) of serum or plasma without the need for extraction or concentration of the sample. With further optimization, even smaller volumes (less than or equal to 10 microliters) can be employed. Sample preparation consisted of precipitation of plasma proteins with absolute ethanol. After centrifugation, a small volume (10 to 50 microliters) of the supernatant was injected directly onto the high performance liquid chromatography (HPLC) column. A dual channel electrochemical detector was used for quantitation of the vitamin E. Pooled serum or plasma was used to determine within-day (8.43 +/- 0.19 mg/L) and between-day (8.49 +/- 0.34 mg/L) precision. Analytical recovery of added vitamin E (4-16 mg/L) averaged 102 +/- 7.0%. No interfering peaks were observed. Linearity was demonstrated over 0.5 to 800 ng. Vitamin E acetate is not detected by this method. HPLC with electrochemical detection is a highly sensitive and specific method for the quantitation of vitamin E in plasma or serum. This procedure is rapid and simple, and can be performed with very small sample volumes.
维生素E缺乏与多种病理状况有关，其中许多涉及新生儿。先前发表的维生素E定量方法需要大量样本以及广泛的提取和浓缩程序。本研究描述了一种无需对样本进行提取或浓缩即可对小体积（小于或等于50微升）血清或血浆中的维生素E进行定量的方法。经过进一步优化，甚至可以使用更小的体积（小于或等于10微升）。样本制备包括用无水乙醇沉淀血浆蛋白。离心后，将一小体积（10至50微升）的上清液直接注入高效液相色谱（HPLC）柱。使用双通道电化学检测器对维生素E进行定量。用混合血清或血浆来确定日内精密度（8.43±0.19毫克/升）和日间精密度（8.49±0.34毫克/升）。添加的维生素E（4 - 16毫克/升）的分析回收率平均为102±7.0%。未观察到干扰峰。在0.5至800纳克范围内显示出线性关系。该方法未检测到维生素E醋酸酯。高效液相色谱结合电化学检测是一种用于定量血浆或血清中维生素E的高灵敏度和特异性方法。该程序快速简单，并且可以用非常小的样本体积进行操作。
