College of Animal Science and Technology, Hebei Agricultural University, Baoding, Hebei 071001, China.
College of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei 071001, China.
Poult Sci. 2023 Oct;102(10):102968. doi: 10.1016/j.psj.2023.102968. Epub 2023 Jul 27.
This study discusses the effects of resveratrol (RES) on the productive performance, immune function and intestinal barrier function of broiler chickens challenged with lipopolysaccharide (LPS). Two hundred and forty 1-day-old male Arbor Acres broilers were randomly divided into 4 groups of 6 replicates each, with 10 broilers per replicate. This experiment used a 2 × 2 factorial design with dietary factors (basal diets or basal diets supplemented with 400 mg/kg RES were administered from d 1 to 21) and stress factors (intraperitoneal injection of 0.5 mg/kg BW of saline or LPS at 16, 18 and 20 d of age). The results showed that LPS challenge had a significant adverse effect on average daily gain (ADG) in broilers at 16 to 21 d of age (P < 0.05), whereas the addition of RES to the diet inhibited the LPS-induced decrease in ADG (P < 0.05). RES also alleviated LPS-induced immune function damage in broilers, which was manifested by the decrease of spleen index (P < 0.05) and the recovery of serum immunoglobulin M and ileal secretory immunoglobulin A content (P < 0.05). The LPS challenge also disrupts intestinal barrier function and inflammation, and RES mitigates these adverse effects in different ways. RES attenuated LPS-induced reduction of villus height in the jejunum and ileum of broilers (P < 0.05). LPS also caused an abnormal increase in plasma D-lactic acid levels in broilers (P < 0.05), which was effectively mitigated by RES (P < 0.05). LPS challenge resulted in a significant decrease in mRNA expression of occludin in the intestinal mucosa (P < 0.05), which was mitigated by the addition of RES (P < 0.05). RES significantly decreased the mRNA expression of toll-like receptor 4, nuclear factor kappa-B and tumor necrosis factor alpha in the ileum tissue stimulated by LPS (P < 0.05). Taken together, this study shows that RES exerts its beneficial effect on broilers challenged with LPS by alleviating immune function damage, relieving intestinal inflammation and barrier damage, and thus improving growth performance.
本研究探讨了白藜芦醇（RES）对脂多糖（LPS）攻毒肉鸡生产性能、免疫功能和肠道屏障功能的影响。将 240 只 1 日龄雄性 Arbor Acres 肉鸡随机分为 4 组，每组 6 个重复，每个重复 10 只。本试验采用 2×2 因子设计，日粮因素（基础日粮或基础日粮添加 400mg/kgRES，121 日龄添加）和应激因素（16、18 和 20 日龄时腹腔注射 0.5mg/kgBW 生理盐水或 LPS）。结果表明，LPS 攻毒对 1621 日龄肉鸡的平均日增重（ADG）有显著的负面影响（P＜0.05），而日粮中添加 RES 抑制了 LPS 引起的 ADG 下降（P＜0.05）。RES 还缓解了 LPS 攻毒对肉鸡免疫功能的损害，表现为脾脏指数下降（P＜0.05）和血清免疫球蛋白 M 和回肠分泌型免疫球蛋白 A 含量恢复（P＜0.05）。LPS 攻毒还破坏了肠道屏障功能和炎症，RES 以不同的方式减轻了这些不利影响。RES 减轻了 LPS 攻毒对肉鸡空肠和回肠绒毛高度的降低（P＜0.05）。LPS 还导致肉鸡血浆 D-乳酸水平异常升高（P＜0.05），RES 有效缓解了这一现象（P＜0.05）。LPS 攻毒导致肠黏膜 occludin mRNA 表达显著降低（P＜0.05），添加 RES 可缓解这一现象（P＜0.05）。RES 显著降低了 LPS 刺激的回肠组织中 toll 样受体 4、核因子 kappa-B 和肿瘤坏死因子 alpha 的 mRNA 表达（P＜0.05）。综上所述，本研究表明，RES 通过缓解免疫功能损伤、减轻肠道炎症和屏障损伤，对 LPS 攻毒肉鸡发挥有益作用，从而改善生长性能。
