Wang Yun, Li Fengdi, Yu Hao, Liao Xianpeng, Liu Kailong, Hu Lihua, Ma Hongmin, Wu Dan, Wei Qin, Ju Huangxian
Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China.
Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China.
Talanta. 2026 Jan 1;296:128433. doi: 10.1016/j.talanta.2025.128433. Epub 2025 Jun 10.
In this study, a highly sensitive biosensor for the detection of carcinoembryonic antigen (CEA) was developed by integrating the excellent properties of a near-infrared electrochemiluminescence (NIR ECL) substance and a double ECL signal quencher. Silver nanosheets (Ag-Cys) exhibiting NIR electrochemiluminescence were synthesized via a simple chemical reaction using AgNO and l-cysteine (L-Cys) as precursors. The incorporation of L-Cys not only reduces photochemical damage but also preserved the antigen-binding activity, facilitating the construction of a high-performance immunosensor. In addition, copper-based metal-organic frameworks coated with sarcosine oxidase (Cu-MOFs/SOx) was synthesized and employed as a double quencher toward Ag-Cys based on electron transfer and resonance energy transfer mechanisms. The resulting sensor exhibited high sensitivity and selectivity, with a wide linear range of 0.00005-100 ng mL and a low detection limit of 43.65 fg mL.
在本研究中，通过整合近红外电化学发光（NIR ECL）物质和双ECL信号猝灭剂的优异特性，开发了一种用于检测癌胚抗原（CEA）的高灵敏度生物传感器。以硝酸银（AgNO）和L-半胱氨酸（L-Cys）为前驱体，通过简单的化学反应合成了具有近红外电化学发光特性的银纳米片（Ag-Cys）。L-半胱氨酸的引入不仅减少了光化学损伤，还保留了抗原结合活性，有助于构建高性能免疫传感器。此外，合成了包覆肌氨酸氧化酶的铜基金属有机框架（Cu-MOFs/SOx），并基于电子转移和共振能量转移机制将其用作Ag-Cys的双猝灭剂。所得传感器具有高灵敏度和选择性，线性范围宽达0.00005 - 100 ng/mL，检测限低至43.65 fg/mL。
