Mason James E, Nie Xiaokai, Coca Daniel, Andrews Peter W
The School of Biosciences, The University of Sheffield, Western Bank, S10 2TN Sheffield, UK.
School of Electrical and Electronic Engineering, The University of Sheffield, Western Bank, S10 2TN Sheffield, UK.
Stem Cell Reports. 2025 Jun 10;20(6):102532. doi: 10.1016/j.stemcr.2025.102532.
Heterogeneity of gene expression is a characteristic of stem cell populations. Here, we review our own findings with human pluripotent stem cells, emphasizing their capacity to occupy distinct metastable substates that influence their differentiation outcomes. Experimental studies, such as single-cell transcriptomics and analyses of marker dynamics, demonstrate the transient and dynamic nature of these substates. Based on a novel approach to model sequences of fluorescent marker distributions over time, we suggest that transitions between these substates may be governed by deterministic chaos in addition to stochastic fluctuations of gene expression, both of which likely contribute to the observed dynamic behavior.
基因表达的异质性是干细胞群体的一个特征。在此，我们回顾了我们自己关于人类多能干细胞的研究发现，重点强调了它们占据不同亚稳态的能力，这些亚稳态会影响它们的分化结果。诸如单细胞转录组学和标志物动态分析等实验研究，证明了这些亚稳态的短暂性和动态性。基于一种对荧光标志物随时间分布序列进行建模的新方法，我们认为除了基因表达的随机波动外，这些亚稳态之间的转变可能还受确定性混沌的支配，这两者可能都对观察到的动态行为有贡献。
