Streng M L, Kottke B W, Wasserman E M, Zecker L, Luong L, Kodandaramaiah S, Ebner T J, Krook-Magnuson E
Department of Neuroscience, University of Minnesota, Minneapolis, MN, USA.
Department of Neuroscience, University of Minnesota, Minneapolis, MN, USA.
Neurobiol Dis. 2025 Sep;213:106991. doi: 10.1016/j.nbd.2025.106991. Epub 2025 Jun 9.
Despite research illustrating the cerebellum may be a critical circuit element in processes beyond motor control, and growing evidence for a role of the cerebellum in a range of neurological disorders, including the epilepsies, remarkably little is known about cerebellar changes during seizures. We therefore implemented a novel method for repeated widefield calcium imaging of the cerebellum in awake, chronically epileptic mice. We found widespread changes in cerebellar Purkinje cell activity during temporal lobe seizures. Changes were noted in the anterior and posterior cerebellum (lobules IV-VII), along the midline (vermis), and both ipsilaterally and contralaterally (in the simplex and Crus I) to the seizure focus. This was true for both overtly behavioral seizures and for hippocampal seizures that remained electrographic only - arguing against cerebellar modulation simply reflecting motor components. Moreover, even brief interictal spikes produced widespread alterations in cerebellar activity. Perhaps most remarkably, changes in the cerebellum also occurred prior to any noticeable change in the hippocampal electrographic recordings. Together these results underscore the relevance of the cerebellum with respect to seizure networks, warranting a more consistent consideration of the cerebellum in epilepsy.
尽管有研究表明小脑可能是运动控制以外的其他过程中的关键神经回路元件，并且越来越多的证据表明小脑在包括癫痫在内的一系列神经系统疾病中发挥作用，但对于癫痫发作期间小脑的变化却知之甚少。因此，我们采用了一种新方法，对清醒的慢性癫痫小鼠的小脑进行重复的宽视野钙成像。我们发现在颞叶癫痫发作期间，小脑浦肯野细胞的活动发生了广泛变化。在小脑的前叶和后叶（小叶IV-VII）、沿中线（蚓部）以及癫痫病灶同侧和对侧（单小叶和 Crus I）均观察到了变化。这在明显的行为性癫痫发作以及仅为脑电图表现的海马癫痫发作中均成立，这表明小脑的调节并非仅仅反映运动成分。此外，即使是短暂的发作间期棘波也会引起小脑活动的广泛改变。也许最值得注意的是，在海马脑电图记录出现任何明显变化之前，小脑就已经发生了变化。这些结果共同强调了小脑与癫痫网络的相关性，这使得在癫痫研究中需要更一致地考虑小脑的作用。
