Alberto-Silva Carlos, de Souza Calvi Gabriela, Dos Santos Feliciano Regiane, Silva-Junior José Antônio, Costa Maricilia Silva
Natural and Humanities Sciences Center, Experimental Morphophysiology Laboratory, Federal University of ABC (UFABC), Rua Arcturus, N° 03, Bloco Delta, 09606-070, São Bernardo Do Campo, SP, Brazil.
Instituto de Pesquisa e Desenvolvimento - IP&D, Universidade do Vale do Paraíba - UNIVAP, Av. Shishima Hifumi, 2911, São José Dos Campos, SP, Brazil.
Lasers Med Sci. 2025 Jun 11;40(1):272. doi: 10.1007/s10103-024-04205-w.
Photobiomodulation (PBM) has been suggested as a potential anti-inflammatory therapy, presenting excellent outcomes for several disorders. Reduction in COX-2 mRNA expression in subplantar and brain tissues by PBM was demonstrated, using the classic model of oedema formation and hyperalgesia induced by Carrageenan (Carr). This work investigated the effect of PBM on mRNA expression of key inflammation-related genes (IL-1β, mPGES-1, mPGES-2 and EP) in subplantar and brain tissues obtained from rats receiving Carr.
The animals were treated with Carr, treated with PBM after 1 h and sacrificed after 1, 3 and 6 h. The light source used was a diode laser, with output power of 30 mW and a wavelength of 660 nm. The laser beam illuminated an area of 0.785 cm, resulting in an energy dosage of 7.5 J/cm, applied for 196 s. IL-1β, mPGES-1, mPGES-2 and EP mRNAs were determined by RT-PCR.
It was observed a reduction in IL-1β expression as in subplantar tissue as in brain parenchyma in animals treated with PBM. In addition, the expression of both mPGES-1 and mPGES-2 mRNA was decreased after PBM.
These results suggested that PBM could reduce the production of IL-1β and subsequently decreasing the effects of PGE2. Therefore, the possible mechanism by which PBM alleviates hyperalgesia could involve its ability to decrease the expression of inflammatory markers in the CNS, reducing the production of PGE in the spinal cord.
光生物调节作用（PBM）已被认为是一种潜在的抗炎疗法，对多种疾病均有良好疗效。利用角叉菜胶（Carr）诱导水肿形成和痛觉过敏的经典模型，已证实PBM可降低足底和脑组织中COX-2 mRNA的表达。本研究探讨了PBM对接受Carr处理的大鼠足底和脑组织中关键炎症相关基因（IL-1β、mPGES-1、mPGES-2和EP）mRNA表达的影响。
动物接受Carr处理，1小时后接受PBM处理，并在1、3和6小时后处死。使用的光源为二极管激光器，输出功率为30 mW，波长为660 nm。激光束照射面积为0.785 cm，能量剂量为7.5 J/cm，照射时间为196秒。通过RT-PCR测定IL-1β、mPGES-1、mPGES-2和EP mRNA。
观察到接受PBM处理的动物，其足底组织和脑实质中IL-1β的表达均降低。此外，PBM处理后mPGES-1和mPGES-2 mRNA的表达均下降。
这些结果表明，PBM可减少IL-1β的产生，进而降低PGE2的作用。因此，PBM减轻痛觉过敏的可能机制可能涉及其降低中枢神经系统中炎症标志物表达、减少脊髓中PGE产生的能力。
