Ali Daoud, Almutairi Bader O, Ahmed Anis, Almarzoug Mohammed H, Alarifi Saud, Alkahtani Saad
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Dose Response. 2025 Jun 10;23(2):15593258251341599. doi: 10.1177/15593258251341599. eCollection 2025 Apr-Jun.
Nalidixic acid has been used as a potent antibiotic drug in the biomedical sciences. In this study, we assessed photochemical properties of nalidixic acid as well as its effect for photocytotoxicity, apoptosis, and genotoxicity on the mouse fibroblast (L929) cell line over the course of 24 h under the ambient UVB intensity. Reactive oxygen species such as singlet oxygen (O), superoxide anion radical (O ), and hydroxyl radical (OH) were measured by photochemical test and photocytotoxicity, apoptosis, and genotoxicity on the mouse fibroblast (L929) cell line were determined by various methods. Furthermore, in UV-B irritated L929 cells, nalidixic acid decreased GSH and raised LPO levels compared to dark control cells. Nalidixic acid caused concentration-dependent toxicological effects ( < 0.05) in L929 cells when exposed to ambient UVB intensity (1.4 mW/cm). DNA fragmentation and caspase-3 activity were observed to be significantly ( < 0.05) activated in L929 cells under co-exposure of UV-B and nalidixic acid. Our results thus confirm that when exposed to UVB rays, nalidixic acid exhibits both phototoxic and photo-genotoxic effects.
萘啶酸在生物医学领域一直被用作一种强效抗生素药物。在本研究中，我们评估了萘啶酸的光化学性质，以及在环境中紫外线B强度下，其对小鼠成纤维细胞（L929）细胞系在24小时内的光细胞毒性、凋亡和遗传毒性的影响。通过光化学测试测量了单线态氧（O）、超氧阴离子自由基（O ）和羟基自由基（OH）等活性氧，并通过各种方法测定了对小鼠成纤维细胞（L929）细胞系的光细胞毒性、凋亡和遗传毒性。此外，在紫外线B照射过的L929细胞中，与黑暗对照组细胞相比，萘啶酸降低了谷胱甘肽（GSH）水平并提高了脂质过氧化（LPO）水平。当暴露于环境紫外线B强度（1.4 mW/cm）时，萘啶酸在L929细胞中引起浓度依赖性毒理学效应（<0.05）。在紫外线B和萘啶酸共同作用下，L929细胞中的DNA片段化和半胱天冬酶-3活性被观察到显著激活（<0.05）。因此，我们的结果证实，当暴露于紫外线B射线时，萘啶酸表现出光毒性和光遗传毒性效应。
