Lutsiv Tymofiy, Fitzgerald Vanessa K, Neil Elizabeth S, McGinley John N, Hussan Hisham, Thompson Henry J
Cancer Prevention Laboratory, Colorado State University, Fort Collins, CO 80523, USA.
Graduate Program in Cell and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA.
Nutrients. 2025 May 28;17(11):1827. doi: 10.3390/nu17111827.
: Metabolic dysregulation underlies a myriad of chronic diseases, including metabolic dysfunction-associated steatotic liver disease (MASLD) and obesity, and bile acids emerge as an important mediator in their etiology. Weight control by improving diet quality is the standard of care in prevention and control of these metabolic diseases. Inclusion of pulses, such as common bean, is an affordable yet neglected approach to improving diet quality and metabolic outcomes. Thus, this study evaluated the possibility that common bean alters bile acid metabolism in a health-beneficial manner. : Using biospecimens from several similarly designed studies, cecal content, feces, liver tissue, and plasma samples from C57BL/6 mice fed an obesogenic diet lacking (control) or containing cooked common bean were subjected to total bile acid analysis and untargeted metabolomics. RNA-seq, qPCR, and Western blot assays of liver tissue complemented the bile acid analyses. Microbial composition and predicted function in the cecal contents were evaluated using 16S rRNA gene amplicon and shotgun metagenomic sequencing. : Bean-fed mice had increased cecal bile acid content and excreted more bile acids per gram of feces. Consistent with these effects, increased synthesis of bile acids in the liver was observed. Microbial composition and capacity to metabolize bile acids were markedly altered by bean, with greater prominence of secondary bile acid metabolites in bean-fed mice, i.e., microbial metabolites of chenodeoxycholate/lithocholate increased while metabolites of hyocholate were reduced. : In rendering mice resistant to obesogenic diet-induced MASLD and obesity, cooked bean consumption sequesters bile acids, increasing their hepatic synthesis and enhancing their diversity through microbial metabolism. Bean-induced changes in bile acid metabolism have potential to improve dyslipidemia.
代谢失调是包括代谢功能障碍相关脂肪性肝病（MASLD）和肥胖症在内的众多慢性疾病的基础，胆汁酸成为其病因中的重要介质。通过改善饮食质量来控制体重是预防和控制这些代谢性疾病的标准治疗方法。纳入豆类，如菜豆，是一种经济实惠但被忽视的改善饮食质量和代谢结果的方法。因此，本研究评估了菜豆以有益健康的方式改变胆汁酸代谢的可能性。：使用来自几项设计相似研究的生物样本，对喂食缺乏（对照）或含有煮熟菜豆的致肥胖饮食的C57BL/6小鼠的盲肠内容物、粪便、肝脏组织和血浆样本进行总胆汁酸分析和非靶向代谢组学分析。肝脏组织的RNA测序、定量聚合酶链反应（qPCR）和蛋白质免疫印迹分析补充了胆汁酸分析。使用16S rRNA基因扩增子和鸟枪法宏基因组测序评估盲肠内容物中的微生物组成和预测功能。：喂食菜豆的小鼠盲肠胆汁酸含量增加，每克粪便排出更多胆汁酸。与这些作用一致，观察到肝脏中胆汁酸合成增加。菜豆显著改变了微生物组成和代谢胆汁酸的能力，喂食菜豆的小鼠中次级胆汁酸代谢产物更为突出，即鹅去氧胆酸/石胆酸的微生物代谢产物增加，而猪去氧胆酸的代谢产物减少。：食用煮熟的菜豆使小鼠对致肥胖饮食诱导的MASLD和肥胖具有抵抗力，它能螯合胆汁酸，增加其肝脏合成，并通过微生物代谢增强其多样性。菜豆引起的胆汁酸代谢变化有可能改善血脂异常。
