Identification and Expression Characteristics of the Cryptochrome Gene Family in .

Cryptochrome is an important class of blue-light receptors involved in various physiological activities such as photomorphogenesis and abiotic stress regulation in plants. In order to investigate the molecular mechanism of blue-light-induced color change in , we screened and cloned the gene encoding the blue-light receptor Cryptochrome. In order to investigate the molecular mechanism of blue-light-induced color change in , we screened and cloned the gene encoding the blue-light receptor Cryptochrome in , and analyzed the expression characteristics of the Cryptochrome gene in under different light intensities, light quality, and temperatures by qRT-PCR. Through homologous cloning, a total of four genes were obtained in the genome, namely, , , , and . Structural domain analyses of the encoded proteins of the four genes revealed that all CsCRYs proteins had the typical photoreceptor structural domain, PRK (protein kinase C-related kinase). Phylogenetic tree analyses revealed that the four genes , , , and could be categorized into three subfamilies, with and clustered in subfamily I, classified in subfamily II, and belonging to subfamily III. All CsCRYs proteins lacked signal peptides and the instability index was higher than 40, among which the isoelectric points of , , and were around five. qRT-PCR analysis revealed that the expression of all four genes was up-regulated at 75 µmol·m·s blue-light illumination for 4 h. In addition, under treatments of different light quality, the expression of genes was significantly higher under blue light than under red light and a mixture of red light and blue light with a light intensity of 1:1; the expression of and was significantly higher in the mixed light of red and blue light than in the single light treatment, while under different temperature gradients, genes were highly expressed under low-temperature stress at -5 °C and 0 °C. This study provides a basis for further research on blue-light-induced color change in and expands the scope of Cryptochrome gene research.