Kong Yining, Liu Changlai, Li Tianshuai, Fang Ji, Liu Guohua
National Key Laboratory for the Development and Utilization of Forest Food Resources, Co-Innovation Centre for Sustainable Forestry in Southern China, Bamboo Research Institute, Nanjing Forestry University, Nanjing 210037, China.
Jiangsu Vocational College of Agriculture and Forestry, Nanjing 210037, China.
Plants (Basel). 2025 May 27;14(11):1637. doi: 10.3390/plants14111637.
Cryptochrome is an important class of blue-light receptors involved in various physiological activities such as photomorphogenesis and abiotic stress regulation in plants. In order to investigate the molecular mechanism of blue-light-induced color change in , we screened and cloned the gene encoding the blue-light receptor Cryptochrome. In order to investigate the molecular mechanism of blue-light-induced color change in , we screened and cloned the gene encoding the blue-light receptor Cryptochrome in , and analyzed the expression characteristics of the Cryptochrome gene in under different light intensities, light quality, and temperatures by qRT-PCR. Through homologous cloning, a total of four genes were obtained in the genome, namely, , , , and . Structural domain analyses of the encoded proteins of the four genes revealed that all CsCRYs proteins had the typical photoreceptor structural domain, PRK (protein kinase C-related kinase). Phylogenetic tree analyses revealed that the four genes , , , and could be categorized into three subfamilies, with and clustered in subfamily I, classified in subfamily II, and belonging to subfamily III. All CsCRYs proteins lacked signal peptides and the instability index was higher than 40, among which the isoelectric points of , , and were around five. qRT-PCR analysis revealed that the expression of all four genes was up-regulated at 75 µmol·m·s blue-light illumination for 4 h. In addition, under treatments of different light quality, the expression of genes was significantly higher under blue light than under red light and a mixture of red light and blue light with a light intensity of 1:1; the expression of and was significantly higher in the mixed light of red and blue light than in the single light treatment, while under different temperature gradients, genes were highly expressed under low-temperature stress at -5 °C and 0 °C. This study provides a basis for further research on blue-light-induced color change in and expands the scope of Cryptochrome gene research.
隐花色素是一类重要的蓝光受体，参与植物的多种生理活动，如光形态建成和非生物胁迫调节。为了研究蓝光诱导[植物名称]颜色变化的分子机制，我们筛选并克隆了编码蓝光受体隐花色素的基因。为了研究蓝光诱导[植物名称]颜色变化的分子机制，我们在[植物名称]中筛选并克隆了编码蓝光受体隐花色素的基因，并通过qRT-PCR分析了隐花色素基因在不同光强、光质和温度条件下在[植物名称]中的表达特征。通过同源克隆，在[植物名称]基因组中共获得了四个隐花色素基因，即[基因名称1]、[基因名称2]、[基因名称3]和[基因名称4]。对这四个基因编码蛋白的结构域分析表明，所有CsCRYs蛋白都具有典型的光感受器结构域PRK（蛋白激酶C相关激酶）。系统发育树分析表明，[基因名称1]、[基因名称2]、[基因名称3]和[基因名称4]这四个基因可分为三个亚家族，其中[基因名称1]和[基因名称2]聚类在亚家族I中，[基因名称3]分类在亚家族II中，[基因名称4]属于亚家族III。所有CsCRYs蛋白都缺乏信号肽，不稳定指数高于40，其中[基因名称1]、[基因名称2]和[基因名称3]的等电点在5左右。qRT-PCR分析表明，在75 μmol·m·s蓝光照射4小时时，所有四个隐花色素基因的表达均上调。此外，在不同光质处理下，隐花色素基因在蓝光下的表达明显高于红光以及光强为1:1的红光和蓝光混合光；[基因名称1]和[基因名称2]在红光和蓝光混合光下的表达明显高于单一光处理，而在不同温度梯度下，隐花色素基因在-5°C和0°C的低温胁迫下高表达。本研究为进一步研究蓝光诱导[植物名称]颜色变化提供了依据，并扩展了隐花色素基因的研究范围。
