Mazzinelli Elena, Messina Marianna, Favuzzi Ilaria, Vincenzoni Federica, Giannoccolo Alessia, Cacciotti Ilaria, Nocca Giuseppina
Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Rome, Italy.
Dipartimento di Ingegneria, INSTM RU, Università degli Studi Niccolò Cusano, via Don Carlo Gnocchi 3, 00166 Rome, Italy.
Materials (Basel). 2025 May 27;18(11):2532. doi: 10.3390/ma18112532.
Drug delivery systems have revolutionized the clinical field by enabling the targeted, controlled, and sustained release of therapeutic agents, minimizing side effects and maximizing efficacy. Among the various drug delivery platforms, polymer-based systems have gained significant attention due to their versatility and biocompatibility. This study investigates the release of dexamethasone and clobetasol propionate from PLA (poly(lactic acid)) fibers in a cellular culture system. The release profiles were analyzed over 1, 6, and 24 h using high-performance liquid chromatography (HPLC), showing a gradual, continuous release, with clobetasol exhibiting slower release due to its poor water solubility. The presence of fibroblasts did not significantly affect the drug release, and the concentrations increased over time. An intracellular recovery test revealed that both drugs entered the cells, although their concentrations were below the limit of quantification (LOQ). Measurements of the remaining drug in the fibers confirmed a sustained release, with no significant difference between conditions with and without cells. These results highlight the potential of PLA fibrous membranes for controlled drug delivery, though further research is needed to optimize release and improve intracellular quantification for more effective therapeutic applications.
药物递送系统通过实现治疗剂的靶向、可控和持续释放，彻底改变了临床领域，最大限度地减少了副作用并提高了疗效。在各种药物递送平台中，基于聚合物的系统因其多功能性和生物相容性而受到了广泛关注。本研究在细胞培养系统中研究了地塞米松和丙酸氯倍他索从聚乳酸（PLA）纤维中的释放情况。使用高效液相色谱（HPLC）在1、6和24小时内分析释放曲线，结果显示药物呈逐渐、持续释放，由于丙酸氯倍他索水溶性差，其释放速度较慢。成纤维细胞的存在对药物释放没有显著影响，且药物浓度随时间增加。细胞内回收试验表明，两种药物均进入了细胞，尽管其浓度低于定量限（LOQ）。对纤维中剩余药物的测量证实了药物的持续释放，有无细胞条件下无显著差异。这些结果突出了PLA纤维膜在可控药物递送方面的潜力，不过还需要进一步研究来优化释放并改善细胞内定量，以实现更有效的治疗应用。
