Department of Chemistry, University La Sapienza, Rome, Italy.
Engineering Department, Niccolò Cusano University, INSTM RU, Rome, Italy.
Nanotechnology. 2021 Feb 26;32(9):095102. doi: 10.1088/1361-6528/abc5f6.
The goal of supporting and directing tissue regeneration requires the design of new, advanced materials, with features like biocompatibility, biodegradability and adequate mechanical properties. Our work was focused on developing a new injectable biomimetic composite material, based on a peptidic hydrogel and calcium phosphates with the aim of mimicking the chemical composition of natural bone tissue. Arg-Gly-Asp-grafted chitosan was used to promote cell adhesion. The obtained composite hydrogel was characterized with differential scanning calorimetry measurements, rheological analysis, field emission scanning electron microscopy, Fourier transform infrared spectroscopy and nuclear magnetic resonance measurements. The biological responsiveness was assessed using the MG-63 human osteoblast cell line.
支持和指导组织再生的目标需要设计具有生物相容性、可生物降解性和适当机械性能等特点的新型先进材料。我们的工作重点是开发一种新的可注射仿生复合材料，该材料基于肽水凝胶和磷酸钙，旨在模拟天然骨组织的化学成分。Arg-Gly-Asp 接枝壳聚糖用于促进细胞黏附。通过差示扫描量热法测量、流变分析、场发射扫描电子显微镜、傅里叶变换红外光谱和核磁共振测量对获得的复合水凝胶进行了表征。通过 MG-63 人成骨肉瘤细胞系评估了生物响应性。
