Kamada M, Daitoh T, Hasebe H, Irahara M, Yamano S, Mori T
Am J Obstet Gynecol. 1985 Oct 1;153(3):328-31. doi: 10.1016/s0002-9378(85)80125-3.
Serum samples with sperm-immobilizing antibody activity from six women were examined for ability to block sperm-egg interaction by a zona penetration test where human follicular ova matured in vitro were used. Exposure of spermatozoa from a fertile healthy donor to the sera impaired binding to and penetration through the zona pellucida of the spermatozoa completely in five cases and incompletely in one case. Successful fertilization in vitro was achieved by using fetal cord serum instead of autoserum of the patient included in the in vitro fertilization and embryo transfer program. These results suggest that interference with sperm-egg interaction may be an additional mechanism of infertility that is caused by antisperm antibodies.
利用体外成熟的人卵泡卵母细胞进行透明带穿透试验，检测了6名具有精子制动抗体活性的女性血清样本阻断精卵相互作用的能力。在5例中，来自健康有生育能力供体的精子与这些血清接触后，完全丧失了与透明带结合及穿透透明带的能力，1例中部分丧失。在体外受精和胚胎移植程序中，使用胎儿脐带血清而非患者自身血清，实现了体外成功受精。这些结果表明，抗精子抗体导致的不育可能存在另一种机制，即干扰精卵相互作用。
