Tsukui S, Noda Y, Yano J, Fukuda A, Mori T
Fertil Steril. 1986 Jul;46(1):92-6.
An in vitro penetration test using human spermatozoa, sera, and eggs stored in a highly concentrated salt solution was designed for examination of the effect of antisperm antibodies on the process of fertilization. Spermatozoa from a healthy fertile donor incubated in modified Biggers, Whiiten and Whittingham (BWW) medium containing 7.5% antisperm-antibody-negative serum, could penetrate through the zonae pellucidae of the stored eggs, but not when the spermatozoa from the same donor had been incubated in modified BWW medium containing 7.5% antisperm-antibody-positive serum. After the antisperm-antibody-positive serum was absorbed with washed spermatozoa, the sperm penetration was not blocked. Therefore, antisperm antibodies appear to block human sperm penetration through the human zona pellucida.
设计了一项体外穿透试验，使用储存在高浓度盐溶液中的人类精子、血清和卵子，以检查抗精子抗体对受精过程的影响。来自健康可育供体的精子在含有7.5%抗精子抗体阴性血清的改良比格斯、惠滕和惠廷厄姆（BWW）培养基中孵育后，能够穿透储存卵子的透明带，但当来自同一供体的精子在含有7.5%抗精子抗体阳性血清的改良BWW培养基中孵育时，则不能穿透。在用洗涤过的精子吸收抗精子抗体阳性血清后，精子穿透未受阻。因此，抗精子抗体似乎会阻止人类精子穿透人类透明带。
