Balouei Fatemeh, Rivera Christina de, Paradis Andrea, Stefanon Bruno, Kelly Stephanie, McCarthy Noelle, Mongillo Paolo
Agrifood, Environmental and Animal Science, University of Udine, Via delle Scienze, 206, 33100 Udine, Italy.
Transpharmation Canada Ltd., Fergus, ON N1M 2W8, Canada.
Animals (Basel). 2025 May 30;15(11):1619. doi: 10.3390/ani15111619.
Gut microbiota composition plays a crucial role in host health and may be influenced by age and disease conditions. This study investigates the gut microbiota diversity of 175 dogs across three age groups (Junior (20-46 months, 43 dogs), Adult (47-92 months, 58 dogs), and Senior (93-168 months, 74 dogs), and examined the impact of osteoarthritis on microbial composition. Alpha diversity analysis using the Shannon and Chao1 indices were significant ( < 0.05) in Senior dogs Beta diversity analysis based on Bray-Curtis dissimilarity indices demonstrated substantial overlap in gut microbiota composition across age groups, with no significant clustering observed ( > 0.05). A second analysis compared the microbiota of 69 healthy dogs and 81 dogs affected by osteoarthritis (OA) in the three classes of age. No significant differences were shown for alpha diversity and beta diversity between healthy and OA dogs. This indicates that aging and osteoarthritis do not induce significant shifts in microbial beta diversity, although high inter-individual variability was noted. Linear Discriminant Analysis (LDA) Effect Size (LEfSe) analysis identified distinct bacterial taxa associated with different age groups. Linear Discriminant Analysis (LDA) Effect Size (LEfSe) analysis identified distinct bacterial taxa associated with different age groups. Junior dogs exhibited enrichment in , Erysipelotrichaceae, and , while Adult dogs were characterized by higher abundances of , , and Ruminococcaceae. Senior dogs had increased representation of and . In OA dogs, , , Clostridiaceae, and were significantly enriched in comparison to healthy dogs, suggesting potential microbiota shifts associated with osteoarthritis. Overall, these findings indicate that gut microbiota diversity varies across different life stages, specific bacterial taxa were differentially enriched in relation to age and OA. This study enhances our understanding of gut microbiota dynamics in dogs and provides insights into potential age- and disease-related microbial signatures.
肠道微生物群组成在宿主健康中起着至关重要的作用，并且可能受到年龄和疾病状况的影响。本研究调查了175只狗在三个年龄组（幼年组（20 - 46个月，43只狗）、成年组（47 - 92个月，58只狗）和老年组（93 - 168个月，74只狗））中的肠道微生物群多样性，并研究了骨关节炎对微生物组成的影响。使用香农指数和 Chao1 指数进行的α多样性分析在老年组狗中具有显著性（<0.05）。基于 Bray - Curtis 差异指数的β多样性分析表明，不同年龄组的肠道微生物群组成存在大量重叠，未观察到显著聚类（>0.05）。第二项分析比较了三个年龄组中69只健康狗和81只患骨关节炎（OA）狗的微生物群。健康狗和患OA狗之间的α多样性和β多样性没有显著差异。这表明，尽管个体间变异性较高，但衰老和骨关节炎并未引起微生物β多样性的显著变化。线性判别分析效应大小（LEfSe）分析确定了与不同年龄组相关的独特细菌类群。幼年组狗在丹毒丝菌科等方面表现出富集，而成年组狗的特征是某些菌属和瘤胃球菌科的丰度较高。老年组狗中某些菌属的代表性增加。与健康狗相比，患OA狗中的某些菌属、梭菌科等显著富集，表明与骨关节炎相关的潜在微生物群变化。总体而言，这些发现表明肠道微生物群多样性在不同生命阶段有所不同，特定细菌类群因年龄和OA而有差异地富集。本研究增进了我们对狗肠道微生物群动态的理解，并为潜在的与年龄和疾病相关的微生物特征提供了见解。
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