Yang Shuai, Zhou Peng, Zhang Weijie, Zhang Yujia, Guo Haiwei, Wei Yingzhu, Wen Xiaoxin, Jian Jichang, Wang Na, Pang Huanying
Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China.
Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524025, China.
Molecules. 2025 May 31;30(11):2418. doi: 10.3390/molecules30112418.
a pathogenic vibrio, is ubiquitous and the most prevalent disease infecting tropical and subtropical mariculture animals in marine and estuarine environments. It presents a major risk to mariculture companies worldwide and can cause serious disease problems in aquaculture. Recent studies have shown that various pathogens employ post-translational modifications (PTMs) to regulate cellular processes. One of the major PTMs is lysine succinylation, which is widespread in eukaryotic and prokaryotic cells. Many basic biological functions of bacteria are associated with the regulation of lysine (K) succinylation (Ksuc). However, little is known about the role of lysine succinylation in pathogenesis. Here, we performed LC-MS/MS analysis of 1271 proteins from to identify 4252 Ksuc modification sites. The modification of S-ribosylhomocysteine lyase (LuxS) and transcription elongation factor GreA proteins by Ksuc was confirmed through immunoprecipitation combined with Western blot, further validating our proteomics results. Bioinformatics study revealed that the identified Ksuc proteins play roles in multiple cellular processes and vital metabolic pathways, including LuxS, biofilm exopolysaccharide biosynthesis protein EpsG, and the general secretory system (Sec systems), and are proteins that regulate bacterial virulence. Generally, this scientific study serves as the basis for additional research on the pathogenic nature of Ksuc in and reveals potential targets that would accelerate the manufacturing of attenuated vaccines.
一种致病性弧菌，广泛存在，是感染海洋和河口环境中热带和亚热带海水养殖动物的最普遍疾病。它给全球海水养殖公司带来重大风险，并可在水产养殖中引发严重的疾病问题。最近的研究表明，各种病原体利用翻译后修饰（PTM）来调节细胞过程。主要的PTM之一是赖氨酸琥珀酰化，其在真核细胞和原核细胞中广泛存在。细菌的许多基本生物学功能都与赖氨酸（K）琥珀酰化（Ksuc）的调节有关。然而，关于赖氨酸琥珀酰化在发病机制中的作用知之甚少。在这里，我们对来自[具体来源未给出]的1271种蛋白质进行了液相色谱-串联质谱（LC-MS/MS）分析，以鉴定4252个Ksuc修饰位点。通过免疫沉淀结合蛋白质印迹法证实了Ksuc对S-核糖基高半胱氨酸裂解酶（LuxS）和转录延伸因子GreA蛋白的修饰，进一步验证了我们的蛋白质组学结果。生物信息学研究表明，鉴定出的Ksuc蛋白在多个细胞过程和重要代谢途径中发挥作用，包括LuxS、生物膜胞外多糖生物合成蛋白EpsG和通用分泌系统（Sec系统），并且是调节细菌毒力的蛋白质。总的来说，这项科学研究为进一步研究[具体弧菌未给出]中Ksuc的致病性质奠定了基础，并揭示了可能加速减毒疫苗生产的潜在靶点。
