Amerio Natalia Soledad, Barengo Marcela Paola, Bich Gustavo Angel, Zapata Pedro Dario, Villalba Laura Lidia, Castrillo María Lorena
Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y, Naturales. Instituto de Biotecnología Misiones "Dra. María Ebe Reca"-InBioMis. Laboratorio de Biotecnología Molecular, Posadas, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Posadas, Argentina.
Environ Microbiol Rep. 2025 Jun;17(3):e70122. doi: 10.1111/1758-2229.70122.
The growing demand for sustainable alternatives to chemical fungicides has driven the development of microbial-based biocontrol strategies. In this study, the native strain Trichoderma koningiopsis LBM116 (Misiones, Argentina) was optimised for the production of mycolytic enzymes (chitinases, β-1,3-glucanases, and proteases) using factorial and response surface experimental designs. Enzyme secretion was increased by more than 250% compared to initial conditions by selecting specific carbon and nitrogen sources and adjusting inoculum and pH parameters. The optimised enzyme formulation improved lettuce seed germination to 86.66% in the presence of the phytopathogen Fusarium sp., under controlled conditions. In seedling trials, it also reduced disease severity and improved growth parameters. These results confirm the dual effect of the enzyme formulation, acting as a biocontrol agent and plant growth promoter. This work highlights the potential of enzyme formulations derived from T. koningiopsis LBM116 as an effective, low-cost, and sustainable alternative for managing phytopathogens in agriculture.
对化学杀菌剂可持续替代品的需求不断增长，推动了基于微生物的生物防治策略的发展。在本研究中，使用析因和响应面实验设计对本地菌株康宁木霉LBM116（阿根廷米西奥内斯）进行优化，以生产溶菌酶（几丁质酶、β-1,3-葡聚糖酶和蛋白酶）。通过选择特定的碳源和氮源并调整接种量和pH参数，与初始条件相比，酶分泌增加了250%以上。在受控条件下，优化后的酶制剂在植物病原体镰刀菌存在的情况下将生菜种子发芽率提高到了86.66%。在幼苗试验中，它还降低了病害严重程度并改善了生长参数。这些结果证实了酶制剂的双重作用，即作为生物防治剂和植物生长促进剂。这项工作突出了源自康宁木霉LBM116的酶制剂作为农业中管理植物病原体的有效、低成本和可持续替代品的潜力。
