Syahputri Vania, Budhy Theresia Indah, Plumeriastuti Hani, Noor Tengku Natasha Eleena Binti Tengku Ahmad
Department of Oral and Maxillofacial Pathology, Faculty of Dental Medicine, Universitas Airlangga, Malaysia.
Department of Immunology, Postgraduate School, Universitas Airlangga, Malaysia.
J Adv Pharm Technol Res. 2025 Apr-Jun;16(2):80-85. doi: 10.4103/JAPTR.JAPTR_12_25. Epub 2025 May 19.
(MO) has been explored for anticancer drug development. However, conventional extract formulations face limitations in drug delivery. Nanoparticles offer a promising alternative due to their small size, enhancing drug selectivity, efficacy, and safety. Therefore, this study aimed to characterize polyvinyl alcohol-based MO nanoparticles (NpMO) and assess their cytotoxicity and anticancer potential. Moreover, NpMO was synthesized using ultrasonication and characterized by its size, functional groups, and surface morphology. Then, an MTT assay was conducted in Vero and HeLa cells, each divided into a control group and five treatment groups (PV1-5 and PL1-5). The treatment groups received NpMO with various doses: 12.5 µg/mL, 25 µg/mL, 50 µg/mL, 100 µg/mL, and 200 µg/mL. The results were represented as OD values and percentage of viable cells, with statistical analysis performed using SPSS version 27. We found that Vero cell viability remained high at 96%, 95%, 93%, 90%, and 82.3% in PV groups, indicating no significant difference between control and PV1-PV4 groups with statistical analysis. Meanwhile, HeLa cell viability decreased to 98%, 92%, 78%, 69%, and 50.2%, with PL5 showing the lowest viability percentage. Statistical analysis confirmed a significant difference between PL5 and the other PL groups. In conclusion, NpMO showed minimal toxicity to Vero cells (>50% viability up to 200 µg/mL) but significantly reduced HeLa cell viability at 50-200 µg/mL, with the strongest effect at 200 µg/mL, indicating a potential anticancer activity.
钼（MO）已被用于抗癌药物的开发研究。然而，传统的提取物制剂在药物递送方面存在局限性。纳米颗粒因其尺寸小，在提高药物选择性、疗效和安全性方面具有很大潜力，成为一种有前景的替代方案。因此，本研究旨在对基于聚乙烯醇的钼纳米颗粒（NpMO）进行表征，并评估其细胞毒性和抗癌潜力。此外，通过超声法合成了NpMO，并对其尺寸、官能团和表面形态进行了表征。然后，在Vero细胞和HeLa细胞中进行了MTT实验，每个细胞系均分为对照组和五个处理组（PV1 - 5和PL1 - 5）。处理组接受不同剂量的NpMO：12.5 µg/mL、25 µg/mL、50 µg/mL、100 µg/mL和200 µg/mL。结果以OD值和活细胞百分比表示，并使用SPSS 27版进行统计分析。我们发现，PV组中Vero细胞活力分别保持在96%、95%、93%、90%和82.3%，经统计分析表明对照组与PV1 - PV4组之间无显著差异。同时，HeLa细胞活力分别降至98%、92%、78%、69%和50.2%，其中PL5组的活力百分比最低。统计分析证实PL5组与其他PL组之间存在显著差异。总之，NpMO对Vero细胞的毒性极小（高达200 µg/mL时活力>50%），但在50 - 200 µg/mL时显著降低HeLa细胞活力，在200 µg/mL时效果最强，表明其具有潜在的抗癌活性。
