Zufry Hendra, Rosdiana Rosdiana, Sucipto Krishna Wardhana, Ekadamayanti Agustia Sukri, Firdausa Sarah
Division of Endocrinology, Department of Internal Medicine, Metabolism, and Diabetes, Thyroid Center, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia.
Division of Endocrinology, Department of Internal Medicine, Metabolism, and Diabetes, Thyroid Center, Dr. Zainoel Abidin Hospital, Banda Aceh, Indonesia.
J Adv Pharm Technol Res. 2025 Apr-Jun;16(2):99-105. doi: 10.4103/JAPTR.JAPTR_388_24. Epub 2025 May 19.
To date, the specific impact of hypoglycemia on insulin resistance in skeletal muscle is unclear. This study aimed to investigate the correlation between hypoglycemia in individuals with Type 2 Diabetes Mellitus by assessing peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and Akt levels in both skeletal muscle tissue and blood plasma. A true experimental-design study was conducted at the Laboratory of Animal Clinical Study, Faculty of Veterinary, Universitas Syiah Kuala, from October to December 2022. The study utilized 24 male rats () and the rates were categorized into the following groups: control (K1), streptozocin-induced diabetic without hypoglycemia (K2), streptozocin-induced diabetic with mild hypoglycemia (K3), and streptozocin-induced diabetic with severe hypoglycemia (K4). The rats were euthanized a minimum of 30 min after hypoglycemia was confirmed. W Quadriceps femoris muscle and 2 ml of heart blood were collected. PGC-1α and protein kinase B/Akt were examined using enzyme-linked immunosorbent assay. This study demonstrates that PGC-1α and Akt levels in skeletal muscle and plasma are influenced by hypoglycemia severity in rats, with lower levels associated with more severe hypoglycemia, though no significant differences were observed between mild and severe hypoglycemia groups. A positive correlation was found between PGC-1α and Akt levels in skeletal muscle and plasma, suggesting interdependency. Control group plasma levels were 2.48 ± 0.53 ng/ml for PGC-1α and 11.26 ± 1.21 ng/ml for Akt. Even mild episodes of hypoglycemia can lead to a substantial deterioration of insulin resistance in skeletal muscle.
迄今为止，低血糖对骨骼肌胰岛素抵抗的具体影响尚不清楚。本研究旨在通过评估2型糖尿病患者骨骼肌组织和血浆中过氧化物酶体增殖物激活受体γ辅激活因子1α（PGC-1α）和Akt水平，来研究低血糖之间的相关性。2022年10月至12月，在亚齐大学兽医学院动物临床研究实验室进行了一项真实实验设计研究。该研究使用了24只雄性大鼠，将其分为以下几组：对照组（K1）、链脲佐菌素诱导的无低血糖糖尿病组（K2）、链脲佐菌素诱导的轻度低血糖糖尿病组（K3）和链脲佐菌素诱导的严重低血糖糖尿病组（K4）。在确认低血糖后至少30分钟对大鼠实施安乐死。采集股四头肌和2毫升心脏血液。使用酶联免疫吸附测定法检测PGC-1α和蛋白激酶B/Akt。本研究表明，大鼠骨骼肌和血浆中的PGC-1α和Akt水平受低血糖严重程度影响，水平越低，低血糖越严重，尽管轻度和重度低血糖组之间未观察到显著差异。在骨骼肌和血浆中，PGC-1α和Akt水平之间发现正相关，表明存在相互依赖性。对照组血浆中PGC-1α水平为2.48±0.53 ng/ml，Akt水平为11.26±1.21 ng/ml。即使是轻度低血糖发作也会导致骨骼肌胰岛素抵抗大幅恶化。
