使用响应面法从[具体来源未给出]中提取和分析抗菌化合物。
Extraction and Analysis of Antimicrobial Compounds from Using Response Surface Methodology.
作者信息
Kazmi Syed Y, Baig Habeeb A
机构信息
Department of Basic Science, College of Medicine, Majmaah University, Majmaah, Saudi Arabia.
Department of Microbiology, College of Medicine, Northern Border University, Arar, Saudi Arabia.
出版信息
J Pharm Bioallied Sci. 2025 May;17(Suppl 1):S865-S868. doi: 10.4103/jpbs.jpbs_1595_24. Epub 2025 Feb 15.
BACKGROUND
Infectious diseases are the world's leading cause of premature mortality, almost killing 65,000 people every day.
OBJECTIVE
The main objective of the study is to find the extraction and analysis of antimicrobial compounds from using response surface methodology.
MATERIALS AND METHODS
ATCC culture samples and antibiotic discs were purchased from PCSIR and scientific markets. All chemicals with reagents of analytical and commercial grades were purchased from Sigma Millipore Chemical Germany, Daejung Chemicals, China, and Unichem England. was purchased from the herbal market and its extracts were prepared in the lab.
RESULTS
The plant extracts; ONO (3.82%) having light green color were arranged and further valued by performing phytochemical analysis of plant extracts. The highest quantity of phenolic content was observed in Ethanolic, Chloroform, and extract of fruit with values 97.0 ± 0.91, 39.5 ± 0.55, and 8.1 ± 0.67 μg GAE/mg extract respectively.
CONCLUSION
It very well might be deduced from this research that in-vitro investigation of the plant showed a critical zone of inhibition against , and .
背景
传染病是全球过早死亡的主要原因，每天导致近65000人死亡。
目的
本研究的主要目的是利用响应面法从[植物名称未给出]中提取和分析抗菌化合物。
材料与方法
ATCC培养样本和抗生素药敏纸片购自巴基斯坦科学与工业研究委员会（PCSIR）和科学市场。所有分析级和商业级的化学试剂购自德国西格玛奥德里奇公司（Sigma Millipore Chemical）、中国大冢化学公司（Daejung Chemicals）和英国联合化学公司（Unichem England）。[植物名称未给出]购自草药市场，其提取物在实验室制备。
结果
植物提取物中，呈浅绿色的ONO（3.82%）被整理出来，并通过对植物提取物进行植物化学分析进一步评估。在乙醇提取物、氯仿提取物和果实提取物中观察到的酚类含量最高，分别为97.0±0.91、39.5±0.55和8.1±0.67μg没食子酸当量/毫克提取物。
结论
从这项研究中可以很好地推断出，对[植物名称未给出]的体外研究显示出对[细菌名称未给出]、[细菌名称未给出]和[细菌名称未给出]的关键抑制区。
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