Kumar B Saravana, Prajapati Ushma H, Das Neelam, Latha K Vel, Shah Vishal S, Mohammadi Arshiya F
Department of Oral Maxillofacial Surgery, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India.
Assistant Professor, Department of Dental and Implant Surgery, Pramukh Swami Medical College, Shree Krishna Hospital, Gujarat, India.
J Pharm Bioallied Sci. 2025 May;17(Suppl 1):S519-S521. doi: 10.4103/jpbs.jpbs_491_25. Epub 2025 Apr 21.
The present study sought to assess, using growth factor release and tissue integration, the healing characteristics of collagen membranes and platelet-rich fibrin (PRF) in guided tissue regeneration (GTR).
The control was commercially available collagen membranes; PRF membranes were produced using a normal centrifugation technique. Scanning electron microscopy (SEM) was used to examine structural features. Key growth factors-including transforming growth factor-beta 1 (TGF-β1), platelet-derived growth factor (PDGF-BB), and vascular endothelial growth factor (VEGF)-were assessed using enzyme-linked immunosorbent assays (ELISA). Using scratch tests, we assessed wound healing capacity and fibroblast migration. The student's -test was used for statistical analysis; < 0.001 was considered to be significant.
While collagen membranes showed a consistent porosity shape, PRF showed a rich fibrin network, including embedded platelets and leukocytes. At 14 days, PRF showed much more cell growth ( < 0.001). Growth factor analysis showed that PRF generated more TGF-β1, PDGF-BB, and VEGF with consistent release ( < 0.001). PRF shows greater healing properties than collagen membranes with enhanced cellular proliferation, movement, and release of growth factors.
These results suggest that PRF might be a more effective biomaterial for use in guided tissue regeneration.
本研究旨在通过生长因子释放和组织整合来评估胶原膜和富血小板纤维蛋白（PRF）在引导组织再生（GTR）中的愈合特性。
对照组为市售胶原膜；PRF膜采用常规离心技术制备。使用扫描电子显微镜（SEM）检查结构特征。使用酶联免疫吸附测定（ELISA）评估关键生长因子，包括转化生长因子-β1（TGF-β1）、血小板衍生生长因子（PDGF-BB）和血管内皮生长因子（VEGF）。通过划痕试验，我们评估了伤口愈合能力和成纤维细胞迁移。采用学生t检验进行统计分析；P<0.001被认为具有显著性。
胶原膜呈现一致的孔隙形状，而PRF呈现丰富的纤维蛋白网络，包括嵌入的血小板和白细胞。在第14天，PRF显示出更多的细胞生长（P<0.001）。生长因子分析表明，PRF产生更多的TGF-β1、PDGF-BB和VEGF，且释放一致（P<0.001）。PRF在细胞增殖、移动和生长因子释放增强方面显示出比胶原膜更强的愈合特性。
这些结果表明，PRF可能是用于引导组织再生的更有效的生物材料。
