Oryzobacter faecalis sp. nov., an Antibiotics-Resistant Actinobacterium of the Family Intrasporangiaceae Isolated from Pig Faeces.

A Gram-stain-positive, aerobic, rod-shaped, and non-motile bacterium, designated strain R7, was isolated from pig faeces. Strain R7 grew at 15-37 °C (optimum 30 °C), pH 6-8 (optimum pH 7), and salinity of 0-5.0% (w/v; optimum 1.0%). Strain R7 was resistant to cefradine, ampicillin, gentamicin, and spectinomycin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R7 belonged to genus Oryzobacter, and showed 98.9% similarity with Oryzobacter terrae PSGM2-16. The average nucleotide identity and digital DNA-DNA hybridization values between strain R7 and O. terrae PSGM2-16 were 84.5% and 27.6%, respectively. The predominant fatty acids of strain R7 were iso-C, Cω8c, iso-C, C, and iso-C. The predominant ubiquinone was MK-8(H). The major polar lipids of strain R7 were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, aminophosphoglycolipid, one unidentified phospholipid, and two unidentified polar lipids. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid. Based on the phenotypic, genotypic and phylogenetic evidence, strain R7 represents a novel species in the genus Oryzobacter, for which the name Oryzobacter faecalis sp. nov. is proposed. The type strain is R7 (=GDMCC 1.4961 = KCTC 59403 = KACC 23902).