He Xinyan, Xiong Miaohuan, Liu Bin, Yan Qiuxiang
College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
College of Life Sciences, Jiangxi Normal University, Nanchang, 330022, China.
Curr Microbiol. 2025 Jun 13;82(8):333. doi: 10.1007/s00284-025-04309-8.
A Gram-stain-positive, aerobic, rod-shaped, and non-motile bacterium, designated strain R7, was isolated from pig faeces. Strain R7 grew at 15-37 °C (optimum 30 °C), pH 6-8 (optimum pH 7), and salinity of 0-5.0% (w/v; optimum 1.0%). Strain R7 was resistant to cefradine, ampicillin, gentamicin, and spectinomycin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R7 belonged to genus Oryzobacter, and showed 98.9% similarity with Oryzobacter terrae PSGM2-16. The average nucleotide identity and digital DNA-DNA hybridization values between strain R7 and O. terrae PSGM2-16 were 84.5% and 27.6%, respectively. The predominant fatty acids of strain R7 were iso-C, Cω8c, iso-C, C, and iso-C. The predominant ubiquinone was MK-8(H). The major polar lipids of strain R7 were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, aminophosphoglycolipid, one unidentified phospholipid, and two unidentified polar lipids. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid. Based on the phenotypic, genotypic and phylogenetic evidence, strain R7 represents a novel species in the genus Oryzobacter, for which the name Oryzobacter faecalis sp. nov. is proposed. The type strain is R7 (=GDMCC 1.4961 = KCTC 59403 = KACC 23902).
从猪粪便中分离出一株革兰氏阳性、需氧、杆状且无运动性的细菌，命名为菌株R7。菌株R7在15 - 37°C（最适温度30°C）、pH 6 - 8（最适pH 7）和盐度0 - 5.0%（w/v；最适1.0%）的条件下生长。菌株R7对头孢拉定、氨苄青霉素、庆大霉素和壮观霉素具有抗性。基于16S rRNA基因序列的系统发育分析表明，菌株R7属于稻田杆菌属，与稻田杆菌PSGM2 - 16的相似性为98.9%。菌株R7与稻田杆菌PSGM2 - 16之间的平均核苷酸同一性和数字DNA - DNA杂交值分别为84.5%和27.6%。菌株R7的主要脂肪酸为异-C、Cω8c、异-C、C和异-C。主要泛醌为MK - 8(H)。菌株R7的主要极性脂为磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰肌醇、氨基磷酸糖脂、一种未鉴定的磷脂和两种未鉴定的极性脂。细胞壁肽聚糖的诊断性二氨基酸为内消旋二氨基庚二酸。基于表型、基因型和系统发育证据，菌株R7代表稻田杆菌属中的一个新物种，为此提出新名称粪稻田杆菌（Oryzobacter faecalis sp. nov.）。模式菌株为R7（=GDMCC 1.4961 = KCTC 59403 = KACC 23902）。
