Yu Hui-Ying, Wei Jia-Tan, Wang Chuan-Xu, Wang Zhuo, Niu Ya-Jie, Li Wen-Jun, Fang Bao-Zhu, Guo Hao-Ran, Li Xin
Shanxi Key Laboratory of Yuncheng Salt Lake Ecological Protection and Resource Utilization, Yuncheng University, Yuncheng 044000, Shanxi, PR China.
College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, Shanxi, PR China.
Int J Syst Evol Microbiol. 2025 Jul;75(7). doi: 10.1099/ijsem.0.006846.
Three Gram-stain-negative, aerobic, rod-shaped, yellow-pigmented bacteria, designated YC-516-91, YC-516-48 and YC-516-93, were isolated from a sediment sample of Yuncheng Salt Lake in China. Growth occurred at 15-42 ℃ (optimum 32-37 ℃), pH 6.0-10.0 (optimum pH 7.0-8.0) and with 1-11.0% NaCl (w/v, optimum 3%). Strain YC-516-91 was positive for hydrolysis of Tween 80 and starch, but negative for nitrate reduction, urease, HS production, hydrolysis of Tween 40 or gelatin. Phylogenetic analysis based on 16S rRNA gene and genome sequences showed that YC-516-91, YC-516-48 and YC-516-93 belonged to the genus . 16S rRNA gene sequence similarity values of YC-516-91 to the closest related type strains MCCC T A10513, 1APP75-27a and 908033 were below 97.99%. Strains YC-516-91, YC-516-48 and YC-516-93 have average nucleotide identity (ANI) values of 99-100%, digital DNA-DNA hybridization values of 92.1-97.4% and average amino acid (AAI) values of 99-100%, suggesting that they belong to the same species. These strains are closely related to MCCC 1A10513, with ANI and AAI of around 92-93%, below the species delineation cut-off, thus indicating that they represent a novel species. The genome of strain YC-516-91 was 2,723,173 bp, and the genomic DNA G+C content was 50.24 mol%. The only respiratory quinone was Q-8, and the major fatty acids (>10%) were iso-C and iso-C. The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipid, glycolipid, unidentified aminolipids and two unidentified polar lipids. Thus, strains YC-516-91, YC-516-48 and YC-516-93 represent a novel species based on their chemotaxonomic, phenotypic and phylogenetic characteristics. Strain YC-516-91 is proposed to represent a novel species named sp. nov., within the genus . The type strain is YC-516-91 (=MCCC 1K09529=KCTC 8778).
从中国运城盐湖的沉积物样本中分离出三株革兰氏阴性、需氧、杆状、产黄色色素的细菌，分别命名为YC-516-91、YC-516-48和YC-516-93。其生长温度范围为15-42℃（最适温度32-37℃），pH值范围为6.0-10.0（最适pH值7.0-8.0），NaCl浓度范围为1-11.0%（w/v，最适浓度3%）。菌株YC-516-91对吐温80和淀粉水解呈阳性，但对硝酸盐还原、脲酶、硫化氢产生、吐温40或明胶水解呈阴性。基于16S rRNA基因和基因组序列的系统发育分析表明，YC-516-91、YC-516-48和YC-516-93属于该属。YC-516-91与最接近的相关模式菌株MCCC 1A10513、1APP75-27a和908033的16S rRNA基因序列相似性值低于97.99%。菌株YC-516-91、YC-516-48和YC-516-93的平均核苷酸同一性（ANI）值为99-100%，数字DNA-DNA杂交值为92.1-97.4%，平均氨基酸同一性（AAI）值为99-100%，表明它们属于同一物种。这些菌株与MCCC 1A10513密切相关，ANI和AAI约为92-93%，低于物种划分阈值，因此表明它们代表一个新物种。菌株YC-516-91的基因组为2,723,173 bp，基因组DNA的G+C含量为50.24 mol%。唯一的呼吸醌为Q-8，主要脂肪酸（>10%）为异-C和异-C。主要极性脂质由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂、糖脂、未鉴定的氨基脂质和两种未鉴定的极性脂质组成。因此，基于其化学分类、表型和系统发育特征，菌株YC-516-91、YC-516-48和YC-516-93代表一个新物种。提议菌株YC-516-91代表该属内一个名为sp. nov.的新物种。模式菌株为YC-516-91（=MCCC 1K09529=KCTC 8778）。
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