Ganbat Dariimaa, Ganbat Sondor, Park Bo Hyeon, Kim Min Kyeong, Sung Jae-Yoon, Chi Won-Jae, Kim Seong-Bo, Lee Dong-Woo, Lee Sang-Jae
Department of Bioscience and Research Center for Extremophiles and Marine Microbiology, Silla University, Busan 46958, Republic of Korea.
Present address: Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.
Int J Syst Evol Microbiol. 2025 Jul;75(7). doi: 10.1099/ijsem.0.006843.
A novel Gram-stain-negative, motile and thermo-halophilic rod-shaped bacterium SMD15-11 was isolated from Seokmodo hot spring in Incheon, Republic of Korea. The strain grew at concentrations of 0.5-7% (w/v) NaCl (optimum at 3%), at pH 5.0-9.0 (optimum at 7.0) and in a temperature range of 30-60 °C (optimum at 50 °C). Strain SMD15-11 shared the highest 16S rRNA gene sequence percentage with 96CJ10356 (92.5%). Both the phylogenetic and phylogenomic analyses showed that strain SMD15-11 clustered with members, demonstrating its close relatedness to the type species of the family. The genome-relatedness indices between strain SMD15-11 and other type species of the family were in the ranges of 67.2-69.1% for average nucleotide identity, 59.3-62.8% for average amino acid identity and 15.9-24.8% for digital DNA-DNA hybridization, which were significantly below the cut-off values for the genus delineation. The genome comprised 3,444,746 bp with a G+C content of 60.1 mol%. The polar lipids contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, four unidentified phospholipids, two unidentified aminophospholipids and two unidentified lipids. The predominant fatty acids are C , C, C and summed feature 3, comprising C C . The polyphasic taxonomic properties indicate that the strain represents a novel genus and species in the family for which the name gen. nov., sp. nov. is proposed. The type strain is SMD15-11 (=KCTC 8289=NBRC 116488).
从韩国仁川石磨岛温泉中分离出一株新型革兰氏阴性、具运动性且嗜热嗜盐的杆状细菌SMD15-11。该菌株在0.5-7%（w/v）NaCl浓度下生长（最适浓度为3%），pH值为5.0-9.0（最适pH值为7.0），温度范围为30-60°C（最适温度为50°C）。菌株SMD15-11与96CJ10356的16S rRNA基因序列相似度最高（92.5%）。系统发育和系统基因组分析均表明，菌株SMD15-11与该科成员聚类，表明其与该科模式种密切相关。菌株SMD15-11与该科其他模式种之间的基因组相关性指数如下：平均核苷酸同一性为67.2-69.1%，平均氨基酸同一性为59.3-62.8%，数字DNA-DNA杂交为15.9-24.8%，均显著低于属划分的临界值。基因组由3,444,746 bp组成，G+C含量为60.1 mol%。极性脂质包含磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、四种未鉴定的磷脂、两种未鉴定的氨基磷脂和两种未鉴定的脂质。主要脂肪酸为C、C、C以及总和特征3（包含C C）。多相分类学特征表明，该菌株代表了该科中的一个新属和新种，为此提出新属名 ，新种名 。模式菌株为SMD15-11（=KCTC 8289=NBRC
