Tadolini B, Cabrini L
Appl Biochem Biotechnol. 1983 Jun;8(3):203-11. doi: 10.1007/BF02778258.
Heparin-sepharose forms complexes with putrescine, spermidine, and spermine and indirect measurements of its affinity for polyamines gives values similar to those obtained with free heparin. A direct measurement of the binding of heparin-sepharose to spermine gives an apparent dissociation constant (Kd) of 1.5 X 10(-6)M spermine. Unlike free heparin, heparin-sepharose does not cause either disruption of the nuclei or more sutble modifications able to modify their sedimentation behavior. The heparin-sepharose polyamine complex formed by the addition of heparin-sepharose to the homogenate can easily be removed and the homogenate can be processed according to normal schedules. Heparin-sepharose is able to sequester 85% of the exchangeable spermine present in the homogenate of rat ventral prostate. The distribution of the marker enzyme galactosyltransferase (Golgi apparatus) on a sucrose density gradient was followed to assess the usefulness of heparin-sepharose in minimizing the aggregation of cellular organelles brought about by polyamines.
肝素-琼脂糖与腐胺、亚精胺和精胺形成复合物，对其与多胺亲和力的间接测量得出的值与游离肝素获得的值相似。对肝素-琼脂糖与精胺结合的直接测量得出精胺的表观解离常数（Kd）为1.5×10⁻⁶M。与游离肝素不同，肝素-琼脂糖既不会导致细胞核破裂，也不会引起能够改变其沉降行为的更细微修饰。通过向匀浆中添加肝素-琼脂糖形成的肝素-琼脂糖多胺复合物可以很容易地去除，匀浆可以按照正常程序进行处理。肝素-琼脂糖能够螯合大鼠腹侧前列腺匀浆中85%的可交换精胺。跟踪标记酶半乳糖基转移酶（高尔基体）在蔗糖密度梯度上的分布，以评估肝素-琼脂糖在最小化多胺引起的细胞器聚集方面的效用。
