Tadolini B, Cabrini L, Sechi A M
Appl Biochem Biotechnol. 1984 Apr;9(2):153-9. doi: 10.1007/BF02798749.
Many experimental systems would greatly benefit from the availability of a simple and effective technique to remove polyamines from biological materials. We have examined the possibility of utilizing heparin-sepharose in the removal of polyamines from rat heart mitochondria, DNA-spermine complex, and fetal calf serum. Heparin-sepharose removes 90% of spermine adsorbed to the cytoplasmic surface of rat heart mitochondria. Heparin-sepharose almost totally removes spermine from DNA-spermine complex, leaving less than 0.003 mol spermine/mol DNA phosphorus. Heparin-sepharose is highly effective in removing spermine and spermidine (99.5 and 95% adsorbed, respectively) from fetal calf serum. Under the same experimental conditions only 50% of putrescine is adsorbed. A higher amount of resin corresponding to an increased capacity for putrescine must be used to achieve a satisfactory removal of putrescine.
许多实验系统将从一种简单有效的从生物材料中去除多胺的技术中大大受益。我们研究了利用肝素琼脂糖从大鼠心脏线粒体、DNA-精胺复合物和胎牛血清中去除多胺的可能性。肝素琼脂糖可去除吸附在大鼠心脏线粒体细胞质表面90%的精胺。肝素琼脂糖几乎能完全从DNA-精胺复合物中去除精胺，使每摩尔DNA磷中残留的精胺少于0.003摩尔。肝素琼脂糖在从胎牛血清中去除精胺和亚精胺方面非常有效（分别吸附99.5%和95%）。在相同的实验条件下，只有50%的腐胺被吸附。必须使用更高量的树脂（对应于腐胺去除能力的增加）才能实现对腐胺的满意去除。
