大麦中赭曲霉毒素A的放射免疫分析
Radioimmunoassay of ochratoxin A in barley.
作者信息
Rousseau D M, Slegers G A, Van Peteghem C H
出版信息
Appl Environ Microbiol. 1985 Aug;50(2):529-31. doi: 10.1128/aem.50.2.529-531.1985.
Abstract
A simple and rapid radioimmunoassay was developed for the quantitative determination of ochratoxin A in barley. [14C]ochratoxin A, with a specific activity of 130 Ci/mol, was used as the tracer. Toxin levels below 100 ng/ml required a cleanup step. Three methods (the Association of Official Analytical Chemists cleanup method, the solvent partition method, and the Extrelut 3 column cleanup method) were compared.
摘要
开发了一种简单快速的放射免疫分析法，用于定量测定大麦中的赭曲霉毒素A。以比活度为130 Ci/mol的[14C]赭曲霉毒素A作为示踪剂。毒素水平低于100 ng/ml时需要进行净化步骤。比较了三种方法（官方分析化学家协会净化法、溶剂分配法和Extrelut 3柱净化法）。
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本文引用的文献
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Enzyme-linked immunosorbent assay of ochratoxin A in wheat.小麦中赭曲霉毒素A的酶联免疫吸附测定
J Assoc Off Anal Chem. 1984 Jan-Feb;67(1):45-9.
2
Enzyme-linked immunosorbent assay of ochratoxin A in barley.
J Assoc Off Anal Chem. 1983 Nov;66(6):1481-4.
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Production of antibody against ochratoxin A.抗赭曲霉毒素A抗体的产生。
Appl Environ Microbiol. 1976 Jun;31(6):831-5. doi: 10.1128/aem.31.6.831-835.1976.
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Balkan (endemic) nephropathy and foodborn ochratoxin A: preliminary results of a survey of foodstuffs.巴尔干（地方性）肾病与食源性赭曲霉毒素A：食品调查的初步结果
Acta Pathol Microbiol Scand B. 1977 Jun;85(3):238-40. doi: 10.1111/j.1699-0463.1977.tb01702.x.
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