Rousseau D M, Slegers G A, Van Peteghem C H
Appl Environ Microbiol. 1985 Aug;50(2):529-31. doi: 10.1128/aem.50.2.529-531.1985.
A simple and rapid radioimmunoassay was developed for the quantitative determination of ochratoxin A in barley. [14C]ochratoxin A, with a specific activity of 130 Ci/mol, was used as the tracer. Toxin levels below 100 ng/ml required a cleanup step. Three methods (the Association of Official Analytical Chemists cleanup method, the solvent partition method, and the Extrelut 3 column cleanup method) were compared.
开发了一种简单快速的放射免疫分析法，用于定量测定大麦中的赭曲霉毒素A。以比活度为130 Ci/mol的[14C]赭曲霉毒素A作为示踪剂。毒素水平低于100 ng/ml时需要进行净化步骤。比较了三种方法（官方分析化学家协会净化法、溶剂分配法和Extrelut 3柱净化法）。
