Harrison Ruby E, Etheridge Ronald Drew
Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA; Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
STAR Protoc. 2025 Jun 13;6(3):103894. doi: 10.1016/j.xpro.2025.103894.
Human infections by Trypanosoma cruzi propagate via its blood-feeding triatomine vector. Investigating parasite-vector interactions depends upon robust techniques to rear insects and analyze infections. Here, we present a protocol for laboratory rearing and infection tracking of Rhodnius prolixus. We describe steps for housing, feeding, and sorting strategies using 3D-printable designs. We also detail procedures for gut dissection, fecal collection, and parasite re-isolation. This protocol describes techniques that support efforts to understand and mitigate vector-mediated Chagas disease transmission.
克氏锥虫引起的人类感染通过其吸血的锥蝽媒介传播。研究寄生虫与媒介之间的相互作用依赖于饲养昆虫和分析感染情况的可靠技术。在此，我们展示了一种用于实验室饲养和追踪罗得西亚锥蝽感染情况的方案。我们描述了使用3D可打印设计进行饲养、喂食和分类策略的步骤。我们还详细说明了肠道解剖、粪便收集和寄生虫重新分离的程序。该方案描述的技术有助于理解和减轻媒介传播的恰加斯病的传播。
