Tareau Anne-Sophie, Tahrioui Ali, Gonzalez Mathieu, Croize Evan, Varin Simon Jennifer, Barreau Magalie, David Audrey, Reffuveille Fany, Brunel Jean-Michel, Lesouhaitier Olivier, Chevalier Sylvie
Univ Rouen Normandie, Université Caen Normandie, Normandie Univ, CBSA UR 4312, F-76000, Rouen, France.
Univ Rouen Normandie, Platform of Sanitary Safety, Evreux Campus, PS2E, France.
Biofilm. 2025 May 27;9:100293. doi: 10.1016/j.bioflm.2025.100293. eCollection 2025 Jun.
is an opportunistic pathogen that causes both acute and chronic infections, including pneumonia, bloodstream infections, urinary tract infections, and surgical site infections. It poses a significant threat to individuals with chronic lung conditions, particularly those with cystic fibrosis. Squalamine and claramine A1 have emerged as promising antibacterial compounds, exhibiting activity against a broad range of both Gram-positive and Gram-negative bacteria. Beyond their potent antibacterial properties, our findings reveal that sub-inhibitory concentrations of claramine A1 and squalamine can disperse pre-formed biofilm without impacting bacterial growth. While claramine A1, but not squalamine, enhances membrane fluidity, the structural difference between these compounds lies primarily in their spermine and spermidine moieties. Notably, we found that spermine, unlike spermidine, was able to both disperse biofilm and increase membrane fluidity. Together, our results suggest that while both compounds are effective at disrupting biofilm, they likely act through distinct mechanisms.
是一种机会致病菌，可引起急性和慢性感染，包括肺炎、血流感染、尿路感染和手术部位感染。它对患有慢性肺部疾病的个体构成重大威胁，尤其是那些患有囊性纤维化的个体。鲨胺和克拉胺A1已成为有前景的抗菌化合物，对多种革兰氏阳性菌和革兰氏阴性菌均有活性。除了其强大的抗菌特性外，我们的研究结果表明，亚抑制浓度的克拉胺A1和鲨胺可以分散预先形成的生物膜，而不影响细菌生长。虽然克拉胺A1而非鲨胺可增强膜流动性，但这些化合物之间的结构差异主要在于它们的精胺和亚精胺部分。值得注意的是，我们发现精胺与亚精胺不同，它既能分散生物膜又能增加膜流动性。总之，我们的结果表明，虽然这两种化合物在破坏生物膜方面都有效，但它们可能通过不同的机制起作用。
