Templeton D M, Sarkar B
Biochem J. 1985 Aug 15;230(1):35-42. doi: 10.1042/bj2300035.
The predominant renal and urinary forms of nickel consist of low-Mr complexes. Similarities in the nature of these complexes have been found in kidneys of rats exposed parenterally to NiCl2 and in rat kidneys treated with NiCl2 in vitro. Similar complexes have also been identified after treatment of bovine and human renal soluble fractions with NiCl2. The bulk of nickel in all cases is associated with sulphated oligosaccharide fractions containing uronic acids and neutral sugars. This binding is non-specific, and nickel is readily displaced from these fractions by copper. Smaller amounts of nickel are bound to an acidic peptide, which was purified from human kidneys and partially characterized. Nickel was not displaced from this material by copper at physiological pH. These nickel complexes have not been found in plasma, suggesting that ligand exchange occurs during or after glomerular filtration of the metal.
镍在肾脏和尿液中的主要存在形式是低分子量复合物。在经肠胃外给予NiCl₂的大鼠肾脏以及体外经NiCl₂处理的大鼠肾脏中，已发现这些复合物性质相似。在用NiCl₂处理牛和人的肾脏可溶性组分后，也鉴定出了类似的复合物。在所有情况下，大部分镍都与含有糖醛酸和中性糖的硫酸化寡糖组分结合。这种结合是非特异性的，在生理pH值下，铜很容易将镍从这些组分中置换出来。少量镍与一种酸性肽结合，该酸性肽是从人肾脏中纯化出来的，并进行了部分特性鉴定。在生理pH值下，铜不能从该物质中置换出镍。在血浆中未发现这些镍复合物，这表明在金属的肾小球滤过过程中或之后发生了配体交换。
