L-抗坏血酸和脱氢-L-抗坏血酸跨肾皮质基底外侧膜囊泡的转运

Transport of L-ascorbic acid and dehydro-L-ascorbic acid across renal cortical basolateral membrane vesicles.

作者信息

Bianchi J, Rose R C

出版信息

Biochim Biophys Acta. 1985 Nov 7;820(2):265-73. doi: 10.1016/0005-2736(85)90120-8.

Abstract

The uptake of L-ascorbic acid and dehydro-L-ascorbic acid into renal cortical basolateral membrane vesicles has been characterized. The uptake systems for both solutes demonstrate saturation kinetics. The presence of structural analogs of L-ascorbic acid and dehydro-L-ascorbic acid results in cis-inhibition and trans-stimulation. Uptake of each substrate is Na+-independent, proceeding to an endpoint of substrate equilibrium across the vesicular membrane. The transport mechanism(s) for L-ascorbic acid and dehydro-L-ascorbic acid appears to be facilitated diffusion.

摘要

已对L-抗坏血酸和脱氢-L-抗坏血酸进入肾皮质基底外侧膜囊泡的过程进行了表征。两种溶质的摄取系统均表现出饱和动力学。L-抗坏血酸和脱氢-L-抗坏血酸的结构类似物的存在会导致顺式抑制和反式刺激。每种底物的摄取均不依赖于Na +，会达到跨囊泡膜的底物平衡终点。L-抗坏血酸和脱氢-L-抗坏血酸的转运机制似乎是易化扩散。