Demchenko A P, Shcherbatska N V
Biophys Chem. 1985 Aug;22(3):131-43. doi: 10.1016/0301-4622(85)80035-1.
Molecular relaxation fluorescence methods were applied to analyze the nature and characteristic times of motions of amphiphilic molecules absorbed in the polar region of a phospholipid bilayer. The fluorescence probes 2-toluidinonaphthalene-6-sulfonate and 1-anilinonaphthalene-8-sulfonate in egg phosphatidylcholine vesicles were studied. The methods of edge excitation fluorescence red shifts, nanosecond time-resolved spectroscopy, fluorescence quenching by hydrophilic and hydrophobic quenchers and emission wavelength dependence of polarization were used. The structural (dipolar) relaxation is shown to be a very rapid (subnanosecond) process. The observed nanosecond phenomena are related to translational movement of the chromophore itself towards a more polar environment and its rotation. The polar surface area of the phospholipid membrane appears to be a highly mobile liquid-like system.
应用分子弛豫荧光方法分析了吸附在磷脂双分子层极性区域的两亲分子运动的性质和特征时间。研究了卵磷脂酰胆碱囊泡中的荧光探针2-甲苯胺基萘-6-磺酸盐和1-苯胺基萘-8-磺酸盐。使用了边缘激发荧光红移、纳秒时间分辨光谱、亲水性和疏水性猝灭剂的荧光猝灭以及极化的发射波长依赖性等方法。结果表明，结构（偶极）弛豫是一个非常快速（亚纳秒）的过程。观察到的纳秒现象与发色团本身向极性更强的环境的平移运动及其旋转有关。磷脂膜的极性表面积似乎是一个高度流动的类液体系统。
