关于细菌闲置马达的旋转布朗运动。II. 荧光相关光谱学理论。

On the rotational brownian motion of a bacterial idle motor. II. Theory of fluorescence correlation spectroscopy.

作者信息

Hoshikawa H, Asai H

出版信息

Biophys Chem. 1985 Aug;22(3):167-72. doi: 10.1016/0301-4622(85)80039-9.

Abstract

The photon flux autocorrelation function of a fluorescent label attached to a bacterial motor shaft is calculated for the case in which the bacterial motor is considered to be actively but idly rotating. It is shown that even when the fluorescent label has a very short lifetime, fluorescence correlation spectroscopy should provide a useful tool for determining the rate of revolution of the bacterial motor under various solution conditions.

摘要

对于细菌马达轴上附着的荧光标记物，计算了在细菌马达被认为处于主动但空转状态下的光子通量自相关函数。结果表明，即使荧光标记物的寿命非常短，荧光相关光谱法仍应是一种用于确定在各种溶液条件下细菌马达转速的有用工具。