Zhang Chaochao, Wang Shuanchi, Shi Da, Zhao Weidong
Departments of Spine, Hebei Province Cangzhou Hospital of Integrated Traditional and Western Medicine, Cangzhou, China.
Hebei Key Laboratory of Integrated Traditional and Western Medicine in Osteoarthrosis Research, Cangzhou, China.
Medicine (Baltimore). 2025 Jun 13;104(24):e42788. doi: 10.1097/MD.0000000000042788.
Glioblastoma is a highly aggressive brain tumor with poor prognosis. Epidemiological studies suggest lifestyle factors, including tea intake, might influence glioblastoma risk. This study investigates the causal relationship between tea intake and glioblastoma risk using 2-sample Mendelian randomization (MR) analysis. Summary data from genome-wide association studies were used for MR analysis, employing single nucleotide polymorphisms (SNPs) associated with tea intake as instrumental variables. The causal effect of tea intake on glioblastoma risk was estimated using inverse-variance weighted (IVW), MR-Egger, weighted mode, and weighted median methods. Heterogeneity and pleiotropy were assessed using Cochran Q test and MR-Egger regression intercept. Forty SNPs were identified as instrumental variables. The IVW method showed no evidence of a causal association between tea intake and glioblastoma risk (OR = 0.6; 95% CI = 0.02-18.21; P = .7768). Supplementary analyses using MR-Egger, weighted median, and weighted mode methods were consistent with the IVW results. Leave-one-out analysis confirmed result stability, and the funnel plot indicated no pleiotropy. This MR analysis does not support a causal relationship between tea intake and glioblastoma risk. Rigorous methods are crucial for assessing causality in observational studies. Further research is needed to explore the impact of tea intake on glioblastoma using diverse study designs.
胶质母细胞瘤是一种侵袭性很强的脑肿瘤，预后较差。流行病学研究表明，包括饮茶在内的生活方式因素可能会影响患胶质母细胞瘤的风险。本研究使用两样本孟德尔随机化（MR）分析来探讨饮茶与患胶质母细胞瘤风险之间的因果关系。全基因组关联研究的汇总数据用于MR分析，采用与饮茶相关的单核苷酸多态性（SNP）作为工具变量。使用逆方差加权（IVW）、MR-Egger、加权模式和加权中位数方法估计饮茶对患胶质母细胞瘤风险的因果效应。使用Cochran Q检验和MR-Egger回归截距评估异质性和多效性。40个SNP被确定为工具变量。IVW方法未显示饮茶与患胶质母细胞瘤风险之间存在因果关联的证据（OR = 0.6；95% CI = 0.02 - 18.21；P = 0.7768）。使用MR-Egger、加权中位数和加权模式方法的补充分析与IVW结果一致。留一法分析证实了结果的稳定性，漏斗图表明不存在多效性。这项MR分析不支持饮茶与患胶质母细胞瘤风险之间存在因果关系。严谨的方法对于评估观察性研究中的因果关系至关重要。需要进一步研究以使用多种研究设计来探索饮茶对胶质母细胞瘤的影响。
