Wang Lingshuai, Shi Lingyan, Zhang Shuhan, Ma Jiping, Zhang Cheng, Chen Huhui, Zhao Mingwen
Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
Institute of Agricultural Applied Microbiology, Jiangxi Academy of Agricultural Sciences, Nanchang, China.
Appl Environ Microbiol. 2025 Jul 23;91(7):e0067925. doi: 10.1128/aem.00679-25. Epub 2025 Jun 18.
As sessile organisms, fungi often encounter various stresses throughout their lifespan, resulting in the overproduction of reactive oxygen species, which impedes the normal growth of fungi. Previously, we revealed that the Swi6B transcription factor is involved in the stress tolerance of . However, the underlying molecular mechanism is unclear. The present study demonstrated that oxidative stress increased the levels of Swi6B. Direct binding of Swi6B to the promoter region of () resulted in increased transcription of and reduced HO levels in overexpressing strains. In addition to increased Swi6B protein, the phosphorylation level of Swi6B was increased by HO treatment. The overexpression of , a mitogen-activated protein kinase that interacts with Swi6B, increased the phosphorylation level of Swi6B, and treatment with HO further enhanced this increase. As a result, both the binding of Swi6B to the gene and tolerance to HO were increased in -overexpressing strains. The present findings revealed that the Slt2-Swi6B-CAT1 pathway responds to oxidative stress and contributes to improving the survival of in adverse environments.IMPORTANCEIn fungi, environmental stress leads to the accumulation of intracellular reactive oxygen species and leads to oxidative stress. Here, we found that the overexpression of the APSES transcription factor enhances tolerance to oxidative stress in . Swi6B binds to the promoter region of , which increases transcription and reduces the HO levels. In addition, the phosphorylation of Swi6B by Slt2 promotes the regulation of by Swi6B. The Slt2-Swi6B-CAT1 pathway is important for the response of to oxidative stress.
作为固着生物，真菌在其整个生命周期中经常会遇到各种应激，导致活性氧的过量产生，这会阻碍真菌的正常生长。此前，我们揭示了Swi6B转录因子参与了[真菌名称未给出]的应激耐受性。然而，其潜在的分子机制尚不清楚。本研究表明，氧化应激会增加Swi6B的水平。Swi6B与[基因名称未给出]的启动子区域直接结合，导致[基因名称未给出]的转录增加，并降低过表达菌株中的HO水平。除了Swi6B蛋白增加外，HO处理还增加了Swi6B的磷酸化水平。与Swi6B相互作用的丝裂原活化蛋白激酶[基因名称未给出]的过表达增加了Swi6B的磷酸化水平，HO处理进一步增强了这种增加。结果，在[基因名称未给出]过表达菌株中，Swi6B与[基因名称未给出]基因的结合以及对HO的耐受性均增加。本研究结果揭示了Slt2 - Swi6B - CAT1途径对氧化应激作出反应，并有助于提高[真菌名称未给出]在不利环境中的存活率。
重要性
在真菌中，环境应激会导致细胞内活性氧的积累并导致氧化应激。在这里，我们发现APSES转录因子[基因名称未给出]的过表达增强了[真菌名称未给出]对氧化应激的耐受性。Swi6B与[基因名称未给出]的启动子区域结合，这增加了[基因名称未给出]的转录并降低了HO水平。此外，Slt2对Swi6B的磷酸化促进了Swi6B对[基因名称未给出]的调控。Slt2 - Swi6B - CAT1途径对于[真菌名称未给出]对氧化应激的反应很重要。
