Toll样受体7（TLR7）和Toll样受体8（TLR8）基因中的X连锁多态性与抵御基孔肯雅热有关。
X-linked polymorphisms in TLR7 and TLR8 genes are associated with protection against Chikungunya fever.
作者信息
Gotay Wilker Jose Perez, Maciel Mariella Sousa Coêlho, Rodrigues Raphael de Oliveira, Cardoso Cynthia Chester, Oliveira Caroline Nobre, Montenegro Artur Fontenelle Lima, Yaochite Juliana Navarro Ueda
机构信息
Universidade Federal do Ceará, Faculdade de Farmácia, Odontologia e Enfermagem, Departamento de Análises Clínicas e Toxicológicas, Fortaleza, CE, Brasil.
Universidade Federal do Rio de Janeiro, Instituto de Biologia, Laboratório de Virologia Molecular, Rio de Janeiro, RJ, Brasil.
出版信息
Mem Inst Oswaldo Cruz. 2025 Jun 13;120:e230224. doi: 10.1590/0074-02760230224. eCollection 2025.
BACKGROUND
Chikungunya virus (CHIKV) causes an infection that leads to the activation of the innate immune response, triggering receptor pathways such as toll-like receptors (TLRs).
OBJECTIVE
The present study aimed to investigate the association of single nucleotide polymorphisms (SNPs) in genes encoding toll-like receptors 3, 7, and 8 and IRF5 in susceptibility to CHIKV infection and persistent joint pain.
METHODS
A case-control study was carried out. The study included 121 symptomatic cases, 29 asymptomatic cases, and 182 healthy controls matched for age and sex. Polymorphisms were identified by TaqMan® SNP Genotyping assays.
FINDINGS
The G allele of the TLR7 variant (rs3853839 G/C) and the G allele of TLR8 (rs3764879 G/C) were associated with protection against CHIKV infection [adjusted odd ratio (OR) = 0.64; p = 0.02 and adjusted OR = 0.54; p = 0.001, respectively]. Moreover, individuals who presented the G allele in the rs3764879 variant have a greater chance of developing the asymptomatic form (adjusted OR =2.88; p =0.004). The development of persistent joint pain was not associated with any investigated SNPs in positive anti-CHIKV IgG individuals.
MAIN CONCLUSIONS
This study identified TLR7 and TLR8 gene polymorphisms as protective factors for Chikungunya infection.
背景
基孔肯雅病毒（CHIKV）引起的感染会导致先天免疫反应激活，触发诸如Toll样受体（TLR）等受体途径。
目的
本研究旨在调查编码Toll样受体3、7和8以及IRF5的基因中的单核苷酸多态性（SNP）与基孔肯雅病毒感染易感性和持续性关节疼痛之间的关联。
方法
开展了一项病例对照研究。该研究纳入了121例有症状病例、29例无症状病例以及182例年龄和性别匹配的健康对照。通过TaqMan® SNP基因分型检测来鉴定多态性。
结果
TLR7变体（rs3853839 G/C）的G等位基因和TLR8（rs3764879 G/C）的G等位基因与预防基孔肯雅病毒感染相关[校正比值比（OR）=0.64；p = 0.02以及校正OR = 0.54；p = 0.001]。此外，在rs3764879变体中呈现G等位基因的个体发生无症状形式的可能性更大（校正OR = 2.88；p = 0.004）。在抗基孔肯雅病毒IgG阳性个体中，持续性关节疼痛的发生与任何研究的SNP均无关联。
主要结论
本研究确定TLR7和TLR8基因多态性为基孔肯雅病毒感染的保护因素。
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