Assis E I T de, Godinho A N, Freire J M O, Lima Neto M F de, Costa J J N, Souza A L P, Monte A P O do, Matos M H T, Sousa A L M de, Silva J R V, Silva A W B
Laboratório de Biotecnologia e Fisiologia da Reprodução, Universidade Federal do Ceará, Sobral, CE, Brasil.
Núcleo de Pesquisa em Experimentação Animal, Universidade Federal do Ceará, Sobral, CE, Brasil.
Braz J Med Biol Res. 2025 Jun 16;58:e14402. doi: 10.1590/1414-431X2025e14402. eCollection 2025.
The present study aimed to evaluate the protective effects of Aloe vera on doxorubicin (DOX)-induced degeneration in ovarian follicles and stromal cells in mice. Mice (n=48) were randomly divided into six groups. The positive control group mice received pretreatment of N-acetylcysteine orally (po), followed by a single intraperitoneal (ip) dose of DOX after 1 h (NAC+DOX). The negative control group mice were pre-treated with saline (po) and administered a single DOX dose (ip) after 1 h (SAL+DOX). The other groups of mice were pre-treated with different concentrations (0.1, 1.0, or 10.0 mg/kg; po) of Aloe vera and then received a single dose of DOX (ip) after 1 h (AV0.1+DOX, AV1.0+DOX, and AV10.0+DOX). The control group received saline po and ip (SAL+SAL). Aloe vera was administered once daily for 3 consecutive days. On the fourth day, the ovaries were processed for histological analysis, immunohistochemistry, and real-time PCR (mRNA for superoxide dismutase (SOD), catalase (CAT), nuclear factor erythroid 2-related factor 2 (NRF2), and tumor necrosis factor-α (TNF-α). Results showed that 0.1 and 1.0 mg/kg Aloe vera protected ovarian follicles and stromal density against DOX-induced degeneration. Furthermore, 0.1 and 1.0 mg/kg Aloe vera reduced TNF-α protein expression and increased NRF2, SOD, and CAT mRNA levels. In conclusion, 0.1 and 1.0 mg/kg Aloe vera had protective effects against DOX-induced degeneration in ovarian follicles and stromal cells in mice.
本研究旨在评估芦荟对阿霉素（DOX）诱导的小鼠卵巢卵泡和基质细胞变性的保护作用。将48只小鼠随机分为六组。阳性对照组小鼠口服N-乙酰半胱氨酸进行预处理，1小时后腹腔注射单次剂量的DOX（NAC+DOX）。阴性对照组小鼠用生理盐水预处理，1小时后腹腔注射单次剂量的DOX（SAL+DOX）。其他几组小鼠用不同浓度（0.1、1.0或10.0mg/kg；口服）的芦荟进行预处理，1小时后腹腔注射单次剂量的DOX（AV0.1+DOX、AV1.0+DOX和AV10.0+DOX）。对照组小鼠口服和腹腔注射生理盐水（SAL+SAL）。连续3天每天给药一次芦荟。在第4天，对卵巢进行组织学分析、免疫组织化学和实时PCR（超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、核因子红细胞2相关因子2（NRF2）和肿瘤坏死因子-α（TNF-α）的mRNA）检测。结果表明，0.1和1.0mg/kg的芦荟可保护卵巢卵泡和基质密度免受DOX诱导的变性。此外，0.1和1.0mg/kg的芦荟可降低TNF-α蛋白表达，并提高NRF2、SOD和CAT的mRNA水平。总之，0.1和1.0mg/kg的芦荟对DOX诱导的小鼠卵巢卵泡和基质细胞变性具有保护作用。
