Heydari Saman, Barzegar-Jalali Mohammad, Heydari Mostafa, Radmehr Afsaneh, Paiva-Santos Ana Cláudia, Kouhsoltani Maryam, Hamishehkar Hamed
Student Research Committee and Department of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Biotechnology Research Center and Department of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Bioimpacts. 2024;14(6):30243. doi: 10.34172/bi.2024.30243. Epub 2024 Mar 4.
Follicular delivery is one of the targeted drug delivery methods aiming to target the hair follicles. The accumulation and retention time of targeted drugs is enhanced when nanoparticles are used as drug carriers. Particle size is one of the important factors affecting the penetration and accumulation of particles in the hair follicles, and there is a controversy in different studies for the best particle size for follicular delivery. Mouse models are mostly used in clinical trials for dermal, transdermal, and follicular delivery studies. Also, it is essential to investigate the reliability of the results between human studies and mouse models.
Curcumin-loaded nanostructured lipid carriers (NLCs), as a fluorescent agent, with three different particle size ranges were prepared using the hot homogenization method and applied topically on the mouse and human study groups. Biopsies were taken from applied areas on different days after using the formulation. The histopathology studies were done on the skin biopsies of both groups using confocal laser scanning microscopy (CLSM). We compared the confocal laser scanning microscope pictures of different groups, in terms of penetration and retention time of nanoparticles in human and mouse hair follicles.
The best particle size in both models was the 400 nm group but the penetration and accumulation of particles in human and mouse hair follicles were totally different even for the 400 nm group. In human studies, 400 nm particles showed good accumulation after seven days; this result can help to increase the formulation using intervals.
The best particle size for human and mouse follicular drug delivery is around 400 nm and although mouse models are not completely suitable for follicular delivery studies, they can be used in some conditions as experimental models.
毛囊给药是旨在靶向毛囊的靶向给药方法之一。当使用纳米颗粒作为药物载体时，靶向药物的积累和保留时间会增加。粒径是影响颗粒在毛囊中渗透和积累的重要因素之一，对于毛囊给药的最佳粒径，不同研究存在争议。小鼠模型主要用于皮肤、透皮和毛囊给药研究的临床试验。此外，研究人体研究和小鼠模型结果之间的可靠性至关重要。
采用热均质法制备了三种不同粒径范围的负载姜黄素的纳米结构脂质载体（NLC），作为荧光剂，并局部应用于小鼠和人体研究组。在使用制剂后的不同天数，从给药部位取活检组织。使用共聚焦激光扫描显微镜（CLSM）对两组的皮肤活检组织进行组织病理学研究。我们比较了不同组的共聚焦激光扫描显微镜图片，观察纳米颗粒在人和小鼠毛囊中的渗透和保留时间。
两种模型中的最佳粒径均为400nm组，但即使对于400nm组，纳米颗粒在人和小鼠毛囊中的渗透和积累也完全不同。在人体研究中，400nm颗粒在七天后显示出良好的积累；这一结果有助于延长制剂的使用间隔。
人和小鼠毛囊给药的最佳粒径约为400nm，虽然小鼠模型并非完全适用于毛囊给药研究，但在某些情况下可作为实验模型使用。
