Alshehri Turki, Alkhalifah Israa, Alotaibi Areeb, Alsulaiman Alaa F, Al Madani Abdullah, Almutairi Basil, Balhaddad Abdulrahman A
Department of Substitutive Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Front Dent Med. 2025 Jun 4;6:1575161. doi: 10.3389/fdmed.2025.1575161. eCollection 2025.
BACKGROUND/OBJECTIVES: plants have a wide range of anti-inflammatory and antimicrobial activities. This study aims to assess the antibacterial effect of water, methanol, and ethanol extracts of against biofilms.
Three extracts of were prepared using water, methanol, and ethanol. Multiple concentrations ranging between 2.93 and 93.75 mg/ml were achieved, alongside a control group with no extract, and incubated with an overnight culture of . In the following day, the total absorbance was measured at 595 nm. Then, the biofilms were fixed and stained with 0.5% crystal violet to measure the biofilm absorbance at 490 nm. One-way ANOVA and Tukey's tests were applied to identify which specific concentrations differed from the control.
methanol and ethanol extracts significantly affected the total absorbance of ( ≤ 0.001) at 46.87 and 93.75 mg/ml. For biofilm inhibition, water extract was effective ( ≤ 0.001) in reducing the biofilm growth at 23.44 (1.34 ± 0.08), 46.87 (1.31 ± 0.15), and 93.75 (1.04 ± 0.07) mg/ml when compared to the control (1.58 ± 0.11). More reduction was observed among methanol and ethanol extracts, as methanol extract significantly ( ≤ 0.001) inhibited the biofilm growth at 23.44 (0.99 ± 0.15), 46.87 (0.12 ± 0.02), and 93.75 (0.09 ± 0.01) mg/ml. Similarly, ethanol extract's biofilm inhibition was observed at the concentrations of 23.44 (0.45 ± 0.12), 46.87 (0.10 ± 0.02), and 93.75 (0.09 ± 0.04) mg/ml.
These findings suggest that possesses antibacterial properties against biofilms, particularly through its methanol and ethanol extracts.
背景/目的：植物具有广泛的抗炎和抗菌活性。本研究旨在评估[植物名称]的水、甲醇和乙醇提取物对[细菌名称]生物膜的抗菌作用。
使用水、甲醇和乙醇制备了[植物名称]的三种提取物。获得了2.93至93.75毫克/毫升之间的多个浓度，同时设置了一个无提取物的对照组，并与[细菌名称]的过夜培养物一起孵育。第二天，在595纳米处测量总吸光度。然后，将生物膜固定并用0.5%结晶紫染色，以测量490纳米处的生物膜吸光度。应用单向方差分析和Tukey检验来确定哪些特定浓度与对照组不同。
甲醇和乙醇提取物在46.87和93.75毫克/毫升时对[细菌名称]的总吸光度有显著影响（P≤0.001）。对于生物膜抑制，[植物名称]水提取物在23.44（1.34±0.08）、46.87（1.31±0.15）和93.75（1.04±0.07）毫克/毫升时有效（P≤0.001），与对照组（1.58±0.11）相比，可减少生物膜生长。在甲醇和乙醇提取物中观察到更多的减少，因为[植物名称]甲醇提取物在23.44（0.99±0.15）、46.87（0.12±0.02）和93.75（0.09±0.01）毫克/毫升时显著（P≤0.001）抑制[细菌名称]生物膜生长。同样，[植物名称]乙醇提取物在23.44（0.45±0.12）、46.87（0.10±0.02）和93.75（0.09±0.04）毫克/毫升的浓度下观察到生物膜抑制作用。
这些发现表明[植物名称]对[细菌名称]生物膜具有抗菌特性，特别是通过其甲醇和乙醇提取物。
