Balhaddad Abdulrahman A, AlSheikh Rasha N
Department of Restorative Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, P.O.Box 1982, Dammam, 31441, Saudi Arabia.
BDJ Open. 2023 Jul 6;9(1):26. doi: 10.1038/s41405-023-00154-8.
There is a significant apprehension in medicine and dentistry concerning the emergence of antibiotic-resistant pathogens, as it composes a significant threat to global health, particularly oral health. The growing concern that oral pathogens may develop resistance against standard preventive measures raises the need for alternative measures to prevent these pathogens' growth without inducing microbial resistance. Therefore, this study aims to assess the antibacterial properties of eucalyptus oil (EO) against two main oral disease pathogens, Streptococcus mutans, and Enterococci faecalis.
S. mutans and E. faecalis biofilms were initiated using brain-heart infusion (BHI) broth supplemented with 2% sucrose with and without diluted EO. After 24 h of biofilm formation, total absorbance was measured via spectrophotometer; then, the biofilm was fixed, stained with crystal violet dye, and measured at 490 nm. An Independent t-test was used to compare the outcomes.
Diluted EO revealed significant total absorbance reduction against S. mutans and E. faecalis compared to the control (p ≤ 0.001). For the biofilm measurement, S. mutans and E. faecalis biofilms were reduced by around 60- and 30-fold, respectively, compared to the group with no EO (p ≤ 0.001).
Based on this study's results, using EO as an organic compound could be considered an adjunctive tool in preventing the growth of oral pathogens causing dental caries and endodontic infection.
医学和牙科领域对抗生素耐药病原体的出现存在重大担忧，因为它对全球健康，尤其是口腔健康构成了重大威胁。人们越来越担心口腔病原体可能对标准预防措施产生耐药性，这就需要采取替代措施来预防这些病原体的生长，同时又不诱导微生物耐药性。因此，本研究旨在评估桉叶油（EO）对两种主要口腔疾病病原体——变形链球菌和粪肠球菌的抗菌特性。
使用补充了2%蔗糖的脑心浸液（BHI）肉汤在有和没有稀释EO的情况下启动变形链球菌和粪肠球菌生物膜。生物膜形成24小时后，通过分光光度计测量总吸光度；然后，固定生物膜，用结晶紫染料染色，并在490nm处测量。使用独立t检验比较结果。
与对照组相比，稀释后的EO对变形链球菌和粪肠球菌的总吸光度有显著降低（p≤0.001）。对于生物膜测量，与没有EO的组相比，变形链球菌和粪肠球菌生物膜分别减少了约60倍和30倍（p≤0.001）。
基于本研究结果，使用EO作为一种有机化合物可被视为预防导致龋齿和牙髓感染的口腔病原体生长的辅助工具。
