Kurt Mürüvvet, Çet Funda
afyon kocatepe üniversitesi.
.
Acta Chim Slov. 2025 Jun 12;72(2):371-381. doi: 10.17344/acsi.2024.9058.
Silver nanoparticles synthesized using Verbascum uschakense extract at a reaction temperature of 60 °C were characterized through multiple analytical techniques. These included ultraviolet-visible absorption spectroscopy, X-ray diffraction analysis, high-resolution transmission electron microscopy, and scanning electron microscopy coupled with energy dispersive X-ray spectroscopy. Ultraviolet-visible spectral analysis revealed a surface plasmon resonance peak at 432 nanometers, indicating the successful formation of silver nanoparticles. Microscopic analyses demonstrated that the nanoparticles were predominantly spherical in shape, with particle sizes ranging between 4 and 14 nanometers. X-ray diffraction analysis confirmed that the silver nanoparticles possessed a face-centered cubic crystal structure, as evidenced by characteristic diffraction peaks at 2θ values of 38.21°, 44.46°, 64.59°, and 77.48°, corresponding to the (111), (200), (220), and (311) planes, respectively. Regarding the biological activities, the antioxidant capacity of the aqueous extract of V. uschakense was evaluated by its ability to scavenge free radicals. At a concentration of 1 milligram per milliliter, the extract demonstrated a 86.93 percent scavenging effect against the stable radical 2,2-diphenyl-1-picrylhydrazyl. In comparison, the silver nanoparticles synthesized from the plant extract exhibited a 60.04 percent scavenging effect at the same concentration. Additionally, the silver nanoparticles showed strong inhibition in the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay, with values comparable to those obtained using the standard antioxidant TroloxThe antimicrobial properties of the biosynthesized silver nanoparticles were examined using the disc diffusion method. The nanoparticles exhibited inhibitory activity against several pathogenic microorganisms, including Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa, Listeria monocytogenes, and fungal species belonging to the Candida genus. These results highlight the potential of V. uschakense-mediated silver nanoparticles as multifunctional bioactive agents with both antioxidant and antimicrobial properties. The study demonstrates that green synthesis offers a sustainable and effective approach to producing nanomaterials with potential applications in biomedical and pharmaceutical fields.
使用绵毛草提取物在60°C反应温度下合成的银纳米颗粒通过多种分析技术进行了表征。这些技术包括紫外可见吸收光谱、X射线衍射分析、高分辨率透射电子显微镜以及结合能量色散X射线光谱的扫描电子显微镜。紫外可见光谱分析显示在432纳米处有一个表面等离子体共振峰，表明银纳米颗粒成功形成。显微镜分析表明，纳米颗粒主要呈球形，粒径在4至14纳米之间。X射线衍射分析证实银纳米颗粒具有面心立方晶体结构，分别对应于(111)、(200)、(220)和(311)平面的2θ值为38.21°、44.46°、64.59°和77.48°的特征衍射峰证明了这一点。关于生物活性，通过绵毛草水提取物清除自由基的能力评估了其抗氧化能力。在浓度为1毫克/毫升时，该提取物对稳定自由基2,2-二苯基-1-苦基肼显示出86.93%的清除效果。相比之下，由植物提取物合成的银纳米颗粒在相同浓度下显示出60.04%的清除效果。此外，银纳米颗粒在2,2'-联氮-双(3-乙基苯并噻唑啉-6-磺酸)自由基清除试验中表现出强烈的抑制作用，其值与使用标准抗氧化剂Trolox获得的值相当。使用纸片扩散法检测了生物合成银纳米颗粒的抗菌性能。纳米颗粒对几种致病微生物表现出抑制活性，包括金黄色葡萄球菌、变形链球菌、大肠杆菌、铜绿假单胞菌、单核细胞增生李斯特菌以及属于念珠菌属的真菌物种。这些结果突出了绵毛草介导的银纳米颗粒作为具有抗氧化和抗菌特性的多功能生物活性剂的潜力。该研究表明，绿色合成提供了一种可持续且有效的方法来生产在生物医学和制药领域具有潜在应用的纳米材料。
