微生物游离DNA检测在儿科头颈部感染患者病原体检测中的效能——一项初步研究。
Efficacy of microbial cell-free DNA testing for detecting pathogens in pediatric patients with head and neck infections-An initial study.
作者信息
Xu Audrey, Miranda Andrea, Lin Hung Fu, Din Taseer, Balakrishnan Karthik, Truong Mai Thy, Ahmad Iram, Meister Kara
机构信息
Program in Human Biology, School of Humanities & Sciences Stanford University Stanford California USA.
Department of Otolaryngology-Head & Neck Surgery Division of Pediatric Otolaryngology Stanford University School of Medicine Stanford California USA.
出版信息
Pediatr Investig. 2025 Jan 6;9(2):185-190. doi: 10.1002/ped4.12462. eCollection 2025 Jun.
Abstract
We investigate the utility of microbial cell-free DNA (mcfDNA) from the blood to predict surgical culture results. In this study, only 26.9% of patients had mcfDNA results that were considered predictive of the causative organism(s). There was a significant burden of contamination in the cohort, emphasizing that dedicated protocols must be followed when running gene-based diagnostics. Healthy patients showed detectable mcfDNA signals, though typically at lower molecules per milliliter for similar pathogens.
摘要
我们研究了血液中微生物无细胞DNA（mcfDNA）用于预测手术培养结果的效用。在本研究中，只有26.9%的患者的mcfDNA结果被认为可预测致病生物体。该队列中存在明显的污染负担，强调在进行基于基因的诊断时必须遵循专门的方案。健康患者显示出可检测到的mcfDNA信号，尽管对于相似病原体，其每毫升的分子数通常较低。
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